Research Of Induce Differentiation Of Dendritic Cells Derived From Hepatocellular Carcinoma By Fuzi Polysaccharices

OBJECTIVE:To investigate whether Fuzi polysaccharice (FPS) induce the differentiation of Peripheral blood mononuclear cells to dendritic cells(DC).METHODS:Lymphocytes are removed form the monocytes derived from peripheral blood of patients with hepatocellular carcinoma. Then monocytes were added into RMPI-1640 culture-medium including 10%foetal bovine serum. High concentration, medium concentration and low concentration of PBS were added into test groups, GM-CSF,IL-4,TNF-a were added into the positive contrast group and nothing were added into in negative contrast group. Half of the culture-medium were changed every two days. In ten days, the amounts of cells were caculated, the shape of cells were observed dynamicly by inverted microscope, cells were scanned by scaning electron microscope, and the surface types of DC (CD80, CD83, CD86) were tested by flow cytometer.RESULTS:Medium concentration group of FPS could induce the differentiation of Peripheral blood mononuclear cells to DC, promote the proliferation of DCs and the expression of CD80,CD86 and CD83. These are no difference between positive contrast group and medium concentration group of FPS. But there are significantly difference from medium concentration group of FPS to high concentration group of FPS, low concentration group of FPS and negative contrast group.CONCLUSION:FPS could induce the differentiation of Peripheral blood mononuclear cells to dendritic cells(DC), promote the proliferation of DC and the expression of mature surface molecule.