| Traditional Chinese medicine is one of practical science which need basic science to prop up substantial support. Western medicine got a significant development depending on modern science and technique. Traditional Chinese medicine should make the best of achievements of modern science and technique to find out support for explaining it's theory and expanding it's clinic.Pattern of syndrome and zheng hou in clinical research of Traditional Chinese medicine are real and dependable. Because of lacking of Standards of objective evaluation, they neither to be completely accredit nor to be deny. Hence, how can we precision record and evaluate pattern of syndrome and zheng hou of Traditional Chinese medicine? How to deal with the relationships between the commonness and individuality in clinical research?Traditional Chinese medicine posses ability of concrete thinking and intuitive thinking, and provided with organism thinking of "unification of human and environment". Today, with the rapid progress of modern science and technique, and changes of human spectrum of disease, heredity and gene, living environment, can we ignore the benefits which science and technique bring to us in diagnosis and treatment of disease and just use the methods of "si wai chuai nei" which set up in ancient dynasty to diagnose and treat disease without any instrument?Traditional Chinese medicine realized rectal cancer from Qin dynasty. After this, the doctor in different dynasty discussed the causes and pathogenesis about rectal cancer. The theory was forward in Jin, Sui and Tang dynasty. The system theory was set up after Song dynasty. Hence, the theory of traditional Chinese medicine in rectal cancer has long way to go.There is no the name of rectal cancer in ancient classical documents. The delineation of rectal cancer scattered in the discussion of "ji ju", "chang xun", "suo gang zhi", "zang yong zhi", "zheng jia" et al. Ancient doctor had realized that it is a systemic disease. They considered the primary causation of disease is deficient of zhen qi, closeness relation to function of "pi wei". The other causes are emotion, diet, exogenous pathogenic factors. The modern tradition Chinese medicine doctor considers that the primary causation of rectal cancer is Yin-yang unbalance, Pi xu, Shen kui, insufficiency of zhen qi are the basic cause, and Shi re, Huo du, Pi zhi are the symptom,they are causes and effects for each other.But, it is difficult to summary the experiences come from different scholars in traditional Chinese medicine because of the effects originated from different knowledge structure and recognition to the diseases of different scholars. Even though the experience summary provides the clue to disciplinarian of syndrome and treatment of rectal cancer to further traditional Chinese medicine clinical research, but it is impossible to accurately summary and quantify them by modern science language and index. These cause the restrain to the experience in repeatability and generality, standardization. These will be a bottleneck to continue development of traditional Chinese medicine.It has been proved that the effectiveness of traditional Chinese medicine and Chinese herbal drugs are positive in Combined Modality Therapy of rectal cancer. Even though there are a lot of clinical researches of traditional Chinese medicine in rectal cancer, and there are positive reports about the clinical treatment of rectal cancer mainly using by traditional Chinese medicine, but they are usually individual case reports, less mass cases reports were reported by accurate design. There are some serious mistakes of statistic analysis in some reports. These lead to the fact that advanced stage patients of rectal cancer become the main population in treatment of traditional Chinese medicine. Total body condition of patients frequently are worse. Even though we can decrease the symptoms and increase life quality, but it is impossible to most of patients to be cured. With the increasing of health care consciousness of people, there are less and less advanced stage patients of rectal cancer, and the advantage of traditional Chinese medicine in following the prognosis of a disease still is not brought into full play. These are caused by the reason that the theory of traditional Chinese medicine can not be explain by modern science theory and the index of traditional Chinese medicine can not be quantified by modern science technology.Tumor dialectical grouping of traditional Chinese medicine is a kind of comprehensiveness summary to elemental of cause of disease, pathogenesis, peculiarity of disease and tendency of disease in different disease stage according to traditional Chinese medicine theory. It is the conclusion to the essence of currently disease, it is the foundation of determining the treatment based on differentiation of symptoms and signs. The index of western medicine reflect the tendency of disease and biology characteristics of tumor. Thus, if we know the relationship between the dialectical grouping of traditional Chinese medicine and index of western medicine, it is going to be possible to provide the evidence of modern science theory to the theory of traditional Chinese medicine, and is going to be helpful to quantified index of traditional Chinese medicine by standard of modern science technology. But it is still needed to be explore how we combine the achievements of modern medicine science technology with the research about the cause and pathogenesis of traditional Chinese medicine and to further expand research and deeply comprehend the theory of the cause and pathogenesis of traditional Chinese medicine by the achievements of modern medicine science technology.The zheng differentiation treatment of traditional Chinese medicine is the key of guide in clinical treatment. "Same disease different treatments, different diseases in same treatment", to prescribe and medication change according to the changes of symptom. The "symptom" of traditional Chinese medicine is different from the disease of western medicine, and the characteristic of traditional Chinese medicine in clinic lead to the result that most clinical research of traditional Chinese medicine which fit to the theory of traditional Chinese medicine are difficult to practice according to design of strict random, matched contral, reduplication standard. The others of researches of traditional Chinese medicine are just clinical cases report and describe or are summaries of therapy experiences. These researches of traditional Chinese medicine are short of reliability coefficient because they are lack of strict science research designed and Quality control approach.Hence, the subject of this research is to provide the method and evidence for further and deeply comprehending the theory of basica theory of traditional Chinese medicine and Assisting clinical treatment by combining to the achievements of modern medicine science technology.γ-syn is one of the synuclein family, mainly localizes to the axons of neurons. The abnormally expression of this protein may be linked to the process of tumorigenesis, stage-specific of tumors and lymph node invasion. The transcriptional deregulation that results in SNCG in cancer cells induce SNCG expression through demethylation. Overexpression ofγ-syn disrupt mitotic check point and allow cells to continue progression in aneuploidy. Suppressed caspase activity disrupting the apoptotic response. Expression and action of MMP2 result in overexpression of MMP gene through MAPK signaling pathways promote cancer cell metastasis.γ-syn is proving to be a potential biomarker in tumor. Studies have been carried out to explore the methods of suppressing cancer metastasis and recurrence by gene control andγ-syn targeting medicine.To investigate the expression ofγ-syn in rectal adenocarcinomas and its correlation with rectal carcinomas. We selected 86 cases rectal cancer patients (n = 86) who had been treated in national medicine centre of colorectal disease of Nanjing municipal traditional Chinese medical hospital and had been diagnosed by pathology from 2005 to 2007. To collect and detect the cancer tissue sample which were surgical ablation. To collect the demography and clinical dates of patients at the same time, including age and gender of patients, distance between tumor edge and anus, CEA level in serum before operation, degree of tumor cell differentiation, situation of lymph nodes metastasis. Immunohistochemistry with anti-γ-syn antibody was performed in 86 cases rectal adenocarcinomas tissues. We discovered that theγ-syn expression was detected in all of rectal adenocarcinomas tissue (86/86). There is no expression difference in gender and age of patients, CEA in serum before operation, differentiation of adenocarcinomas, length from anus to rectal adenocarcinomas edge and metastasis situation of lymph nodes.γ-syn is highly expressed in rectal adenocarcinomas. Our findings suggest thatγ-syn is a ubiquity express protein and it may represent a biological marker of rectal adenocarcinomas. There is no paper to reportγ-syn expression in rectal adenocarcinomas in China.To investigate the expression regulate ofγ-syn in rectal adenocarcinomas, we chose 22 fresh tissue samples, both of rectal adenocarcinomas and normal mucosa. Detected the SNCG methylation by methylation-specific PCR(MSP). We find that SNCG methylation is common situation in rectal adenocarcinomas. It is suggest that the regulate of SNCG associated to unique CpG island hypomethylation rather than whole gene hypomethylation.To explore whether there are some internal regularity relationship and associativity between the common Zheng hou and distribution regularity of traditional Chinese medicine and index of Western medicine in rectal cancer. We selected 71 cases rectal cancer patients (n= 71) who had been performed operation in national medicine centre of colorectal disease of Nanjing municipal traditional Chinese medical hospital and had been diagnosed by pathology, fitting to the internalization standard from 2004 to 2006. Then numbered those patients. Complete The Chinese and Western medicine index before operation, and define pathological type after operation then proceed Associativity analysis. We discovered that according to the differentiation of symptoms and signs of classification of syndrome of traditional Chinese medicine , QI-blood stagnant existed was the most common type in Dukes B,C stage. The thinness and greasy tongue fur and unsmooth pulse are the common signs in these patients. Weight in the normal range was found in 70.4% patients, overweight was found in 25.4 % patients. There is not Asthenia syndrome patient. The average distance from edge of rectal cancer to anus was (7.2±4.9cm) in QI-blood stagnant type patient, significantly exceeded the type of Shi Re Du Yun patients (4.6±2.0cm). There is statistics difference(P < 0.01). This research discovered that most of rectal cancer patients belong to QI-blood stagnant and Shi Re Du Yun in classification of syndrome of traditional Chinese medicine (Dukes B. C stage). The distance from edge of rectal cancer to anus in QI-blood stagnant type patients was significantly exceeded the type of Shi Re Du Yun type patients. These suggest that it is more possible to process the sphincter perservation operation in the type of QI-blood stagnant type patients. There is similar distribution in index between Western medicine and Traditional Chinese medicine. They can be evidence for each other. We did not find the Asthenia syndrome patient. We think that it is caused by different effect to total body situation between rectal cancer patients and colon cancer patients. The intake of nutrient substance is decreased in early stage of colon cancer patients so that the patients have sign of Asthenia syndrome. To rectal cancer patients, The intake of nutrient substance is decreased in advanced stage, the sign of Asthenia syndrome can be found only in advanced stage. We suggest that it is necessary to classify colon cancer and rectal cancer in diagnose and treatment of traditional Chinese medicine, which would make diagnose and treatment are more accuracy.To explore the relationship and associativity between the common Zheng hou and distribution regularity of traditional Chinese medicine and tumor biological marker of Western medicine in rectal cancer. Whether we can find out the regularity and variation of Zheng hou and Zheng xing of traditional Chinese medicine while the index of western medicine can be detected in early stage of rectal cancer? And to provide the evidence for traditional Chinese medicine in treatment of no symptom rectal cancer, so as to the advantage of following the prognosis of a disease of traditional Chinese medicine bring into full play?We selected 71 cases rectal cancer patients (n = 71) who had been performed operation in national medicine centre of colorectal disease of Nanjing municipal traditional Chinese medical hospital and had been diagnosed by pathology, fit to the internalization standard from 2004 to 2006.Then numbered those patients. To complete The traditional Chinese medicine index before operation and to define pathological type ,detected the expression ofγ-syn after operation then proceed Associativity analysis. We discovered that the expression ofγ-syn in QI-blood stagnant group and Shi Re Du Yun group in classification of syndrome of traditional Chinese medicine are no statistics difference(P=0.54). There is not statistics difference between the overweight group and no overweight group in the expression ofγ-syn(P= 0.82 > 0.05). There is statistics difference between the smooth greasy tongue fur group and thin greasy tongue fur group in the expression ofγ-syn(P=0.03 < 0.05). There is statistics difference between the string unsmooth pulse group and string slippery pulse group in the expression ofγ-syn(P=0.03 < 0.05). we conclude that there is a tendency to smooth greasy tongue fur and thin greasy tongue fur, the string unsmooth pulse and string slippery pulse which maybe have consult effect for diagnosis and treatment of traditional Chinese medicine in rectal cancer while it combine to other index. But if there is a relationship and associativity between the common Zheng hou and distribution regularity of traditional Chinese medicine and tumor biological marker of Western medicine in rectal cancer still need larger sample, multicentre randomised control trial to prove.We can conclude in behalf of this study: 1.γ-syn is a ubiquity express protein and it may represent a biological marker of rectal adenocarcinomas. There is no paper to reportγ-syn expression in rectal adenocarcinomas in the china. 2. Most of rectal cancer patients belong to QI-blood stagnant and Shi Re Du Yun in classification of syndrome of traditional Chinese medicine (Dukes B. C stage). The distance from edge of rectal cancer to anus in QI-blood stagnant type patients was significantly exceeded the type of Shi Re Du Yun type patients. These suggest that it is more possible to process the sphincter perservation operation in the type of QI-blood stagnant type patients.3. We did not find the Asthenia syndrome patient,it is necessary to classify colon cancer and rectal cancer in diagnose and treatment of traditional Chinese medicine, which would make diagnose and treatment are more accuracy. 4. There is statistics difference between the smooth greasy tongue fur group and thin greasy tongue fur group in the expression ofγ-syn(P=0.03 < 0.05) and statistics difference between the string unsmooth pulse group and string slippery pulse group in the expression ofγ-syn(P=0.03 < 0.05). There is a tendency to smooth greasy tongue fur and thin greasy tongue fur, the string unsmooth pulse and string slippery pulse which maybe have consult effect for diagnosis and treatment of traditional Chinese medicine in rectal cancer while it combine to other index. But if there is a relationship and associativity between the common Zheng hou and distribution regularity of traditional Chinese medicine and tumor biological marker of Western medicine in rectal cancer still need larger sample, multicentre randomised control trial to prove.5. SNCG methylation is common situation in rectal adenocarcinomas. It is suggest that the regulate of SNCG associated to unique CpG island hypomethylation rather than whole gene hypomethylation.After finished this study, we provide a new research method that if we can find the relationship between the common Zheng hou of traditional Chinese medicine and index of modern science technique for quantificated the index of traditional Chinese medicine by index of modern science technique, then standardize the diagnosis and treatment of traditional Chinese medicine by studying the relationship between Zheng hou of traditional Chinese medicine and index of western medicine and the relation between Zheng hou of traditional Chinese medicine and tumor biological marker. The subject is to find out the secret of theory of ancient traditional Chinese medicine, and to extend and enlarge the diagnosis information of traditional Chinese medicine in order to help the supplement and consummate of theory system of ancient traditional Chinese medicine and diagnosis and treatment of traditional Chinese medicine.In conclusion, to further studying the relationship between index of traditional Chinese medicine and index of western medicine in rectal cancer patients has important effect to explain theory system of ancient traditional Chinese medicine by theory of modern science technique, quantificating the index of traditional Chinese medicine by index of modern science technique, and guiding clinic treatment and prognosis of traditional Chinese medicine. It is helpful to get the message of the distribution of common Zheng hou of traditional Chinese medicine in rectal cancer and the relationship with the index of modern science technique. It is helpful for the supplement and consummate of theory system of ancient traditional Chinese medicine. It is helpful for quantificated the index of traditional Chinese medicine and standardize the diagnosis and treatment of traditional Chinese medicine. It is important to explain the theory of ancient traditional Chinese medicine using modern medical theory by language of modern science technique.
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