The Treament Of Tonifying Kidney & Regulating Blood And Dispersing Muddy To Resist The Fibrotic Kidney Of Adriamycin In Rat And 2-3 Stage Of Chronic Kidney Disease Of Clinical Curative Effect

Objective:Fibrotic kidney is a common characteristic during the course from chronic kidney disease to chronic renal failure(CRF).The pathological feature of fibrotic kidney is progressing extracellular matrix accumulation.The result is the loss of normal structure of the glomerulus,the destroy of nephron and the deprivation of renal function.The treatment of tonifying kidney & regulating and dispersing muddy(TKRDM) is a kind of means which is set up by professor sun wei.The study includes two parts.First we establish an animal model of renal sclerosis by adriamycin(ADM).Then we use the TKRDM to treat the animal and observe the change of biochemical indicator,the change of histopathology,the change of ECM,the change of cytokine and the marker of cell.Second,we use the TKRDM to treat the patient by 2-3 stage chronic renal disease.Approaches:Research study:1.We did once-intravenous injections to establish an animal model for diffusion mesangioproliferation following with focal segmental glomerulosclerosis.The rats were randomly divided into sham group,adriamycin nepropathy(AN),TKRDM group, benazepril group,TKRDM and benazepril group.Urinary protein excretion,serum urea nitrogen,serum creatinine,albumin,serum cholesterol and triglyceride were be detected by automatic biochemistry meter.We detected the cytokine and the marker of cell,which includes collagenâ…£(Coâ…£),fibronectin(FN),the transforming growth factor-β₁,by the means of immunohistochemistry and molecular biology.2.We would analyse the date by the statistics software.Clinic study:1.We set up the diagnostic criteria and therapeutic principle according to Clinical practice guide for the chronic renal disease and dialysis which was compiled by American NKF-K/DOGI group and Clinical practice guide to the new medicine of TCM which was compiled by the National Food and Medicine Administration Bureau.2.We used the TKRDM to treat the patient by 2-3 stage chronic renal failure and concluded the Urinary protein excretion,the serum urea nitrogen and calculated the date of renal function.3.We analysed the date by the SPSS15.0 statistics software.Result:TKRDM and benazepril have significantly difference in proteinuria extract and urinary NAG enzyme,the serum total cholesterol was significantly distinguished by statistic means. TKRDM can improve the circulating of the blood but the benazepril doesn't have the effect.We can get a significant conclusion by statistic ways.We observed the tissue of the kidney by the way of pathohistology.We could find that the intercapillary cells and the ECM had obviously proliferation in the group of the AN.There were many foam cells in glomerulus of kidney.The cell of the renal tubule had vacuolar degeneration,analosis and cellular necrosis.There were also many protein cast and RBC cast in the nephric tubule.TKRDM group,benazepril group, TKRDM and benazepril group had different degree lessens for the index from the pathohistology. We found the group of the TKRDM and benazepril had the best conclusion by statistic way.We tested the FN,TGF-β1,MMP-9 and Col-â…£which were the compositions correlating with the fibrotic tissue by immunohistochemistry.Then we analyzed the compositions in the payhofigure by the semiquantitative analysis.TKRDM group,benazepril group,TKRDM and benazepril group could restrain the overexpression of the cytokine for the AN.In the mechanism of molecular biology,we used the ways of reverse transcription polymerase chain reaction(RT-PCR).We analyzed the cytokines which included TIMP,TGF-β1,Nephrin.All of the cytokines had a high of the date in the group of AN.The others of experimental groups had a relative lower dates.We used the TKRDM to treat patients.Then we write down the symptoms and the renal function every month.All the symptoms,were selected from Clinical practice guide for the chronic renal disease and dialysis and Clinical practice guide to the new medicine of TCM,which correlate with the chronic renal disease.We analyzed all of the date which come from the symptoms and the renal function by spss15.0 statistic software.Conclusion:From the dates and the analyzing of the experiments,we could get the results. First TKRDM can decrease the proteinuria and protect the function of the renal tubular for AN in rats.Second TKRDM can reduce the proliferation of ECM at different levels for AN in rats.If we use the TKRDM and benazepril,the effects of the therapeutic action will be better than use any other one of them.For the chronic study,TKRDM can tonify spleen and kidney,benefit vital energy and smooth the blood vessel.TKRDM can also elevate the function of the entrails.The experiment hints the two points by analyzing the date of statistic results.First the TKRDM can decrease proteinuria extract and urinary NAG enzyme.Second the TKRDM can delay the aggravate speed of the renal function.