| Research the Relevantof Ruomai and RuobingwithExtracellular Matrix from IPFSpeciality:Basic Theory of TCMAuthor:Zou WanchengTutor:Zhang Liutong;Qiu XingfuanObjective:Study the relevance of ECM deposition with progressivedevelopment of the disease,the mechanismsof Yiqitongluo Decoctiondelaying or blockingprogressive development in IPF fibrosisphase,and discuss the relevance of ECM with Luomai and Luobing.Methods:(1) Theoretic discussion:Collecting essence abstract,eliminateing the false and retaining the true,using of thecomparative research methods of seeking common ground whilereserving differences,on the basis of summing up the traditionalchinese medicine connotation of Luomai and Luobing,the role of ECMdeposition in IPF fibrosis phase,to explore the relevance of ECMwith Luomai and Luobing,pathogenesis mechanisms of traditionalchinese medicine in IPF fibrosis phase,and to seek the theoreticalfoundation of traditional Chinese medicine to combat pulmonaryfibrosis.(2) Experimental Study:①Toestablishamousemodel ofpulmonary fibrosis;②To observate behavior change of mouse modelof pulmonary fibrosis from respiratory rate,bodyweight,etal;③Quantitative analysis of pathological histopathological changes inmouse.④Using immunohistochemical method to detect coll-1,FN,integrinα5β1,TGF-β1,bc1-2,bax in the lung tissuedistribution,using integral optical density method to detectcoll-1,FN,integrinα5β1,TGF-β1,bcl-2,bax in the pulmonary interstitial spaceof the positive IOD values,toexplore the mutualrelations of thecoll-1 andFNwith integrinα5β1,TGF-β1,bcl-2,bax in lung interstitium.Results:(1) behavior study:Chinese medicine treatment group hasa marked improvement comparing groups modeled at the respiratoryrate,weight gain,lung coefficient,P＜0.05.(2)Pathomorphology:Model 1 has mild to moderate lung alveolitis,there are a numberof inflammatory cell infiltration,capillary congestion in lungalveolar space and interstitial lung,Punctate even flakyhemorrhage,a large number of interstitial fibroblasts and collagenfiber deposition,was in moderate to severe pulmonaryfibrosis,with structure disorder in part of the alveoles.inflammation in model 2 Group is sligher than the model 1 Group,P＜0.05,but severe in fibrosis,P＜0.05.Inflammation is thelightest in traditional chinese medicine treatment group,respectively compare with othe groups modeled,P＜0.05;fibrosiscompared with the model 1 was no significant difference,P＞0.05,compared with model 2,there is amarked improvement,P＜0.05.Thereare not difference between Western medicine treatment group andmodel 2 group in the inflammation,the degree of fiber,graydistrict.(3)Immunohistochemistry detectionresults of OID value ofColl-1,FN in lung interstitium:The groups modeled can be seeninterstitial Coll-I showed focal,patchy,cord-like distribution,was significantly increased comparing with sham-operated group,P＜0.01,sedimentary in model 2 is more severe than in model 1 Group,P＜0.05;There are no significant change between Chinese medicinetreatment group and model 1 group,P＞0.05;Western medicinetreatment group compared with the model 2 group,There are no significant improvement,P＞0.05;Chinese medicine treatment groupwas improved compared with the model 2,P＜0.05.FN in lung tissuesof Sham-operated group mice is mainly distributed in the bloodvessels around the bronchi,alveoli and alveolar capsule openinginterval,were tenuous fibrosis-like.FN in chinese medicinetreatment group were similar to in sham -operated group,there arepositive expression at stromal cells in model 1 group,model 2 group,western medicine group,there are also positive staining of FN inECMdeposition,showing irregular,linar,more sparse distribution,comparing among the there gropes,was no significant difference,P＞0.05;separately comparing the three groups with sham-operatedgroup,traditional Chinese medicine group,There are significantdiffrence,P＜0.01.(4)Immunohistochemistry detection results ofOID value of integrinα5β1 in lung interstitium:sham-operatedgroup compared with the traditional Chinese medicine treatmentgroup,P＜0.05,there are significant difference;with othergroups,P＜0.01.Model 2 group is slighter than model 1 group,P＜0.05;Chinese medicine treatment group is slighter than model 1 group,model 2 group and western medicine group,P＜0.05.(5)Imunohistochemistry detection results of OID value of integrinα5β1 in lung interstitium:sham-operated group compared with thetraditional Chinese medicine treatment group,P＜0.05,there issignificant difference;with other groups,P＜0.01;Model 2 Groupshow decrease contrast model 2 Group,P＜0.05;Chinese medicinetreatment group show decrease contrast model 1 group,model 2 group,and western medicine group,P＜0.05.(6) Imunohistochemistrydetection results of OID value of bc1-2 in lung interstitium:sham-operated group compared with TCM treatment group show difference,P＜0.05,with the rest of groups,P＜0.01.Model 2 groupwas enhanced contrasting model 1 group,P＜0.05,similar to thewestern medicine treatment group,P＞0.05.Chinese medicinetreatment group were reduced contrasting model 1 group,model 2group,western medicine treatment group,P＜0.01.Imunohistochemistry detection results of OID value of bcl-2 in lunginterstitium:traditional Chinese medicine treatment grouprespectively compared with the model 1 group,model 2 group,westernmedicine group show difference,P＜0.05.(7)the relationship ofcoll-1,FN with integrinα5β1,TGF-β1,bcl-2,bax in lunginterstitium:there are respectively positivelinear relationshipsbetween co11-1,FN andα5β1,TGF-β1,bcl-2,there are negativelinear relationships between co11-1,FNwithbax inmodel 1 group,model 2 group,western medicine,there are no linear relationshipsbetween co11-1,FN withα5β1,TGF-β1,bcl-2,bax in TCMtreatment group.Conclusion:The Theoretic discussion and experimental results showthat:(1)asa themicro-environmentofcellsurvival,ECM' schangesare not merely passive results inavarietyof stimuli,through theintegrin-mediated it canalso affect the function of cells,playsan important role in progressive development of IPF fibrotic period.The pathological changes caused by it are hightly Consistent withLuobing,it is one of the Basic material of modern biology of Luobing.(2) Yiqitongluo Decoction can effectively inhibt progressivedevelopment of bleomycin-induced pulmonary fibrosis of mouse,itsmechanism is probably blocking the mutal promoting relation betweenECM deposited and integrins.The results of the treatment isconsistent with"recovering Luomai' s functions of the"running and infiltrating Qi and blood,running and Excreting poison,linking from top to bottom and inside to outside",so blockingthe mutal promoting relation between ECM deposited and integrinsmaybe is one connotation of the modern biology of Tongluo law.Subject words pulmonary fibrosis;progressivedevelopment;ECM;Luomai and Luobing;Yiqitongluojiedu...
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