The Effect Of Hydroxysafflor Yellow A On The Proliferation And Differentiation Of 3T3-L1 Preadipocytes And Activity Of Regulatoy Enzymes In Lipid Metabolism

Objective:Obesity has already been a worldwide epidemic disease and it can lead patients to lots of serious diseases,such as coronary heart disease,stroke,hypertension,type 2 diabetes,hyperlipidemia,sleep apnea,and et al.Excess energy intake and stored in the white adipocytes,as well as the proliferation and differentiation of preadipocytes are the two core factors in the development of obesity.Our previous study showed that the main component of Carthamus tinctorius L.,hydroxysafflor yellow A could modulate the activity of PPARγ,a key transcription factor during preadipocyte differentiation.And the result showed the possible roles of hydroxysafflor yellow A in adipocytes.The aim of this study is to learn the effect of hydroxysafflor yellow A on the proliferation and differentiation of 3T3-L1 preadipocyte as well as the activity of fatty acid synthase and hormone sensitive lipase.Methods:1.To study the effect of hydroxysafflor yellow A on the proliferation of 3T3-L1 preadipocytes by MTT detection.2.To study the effect of hydroxysafflor yellow A on the differentiation of 3T3-L1 preadipocytes by the means of Oil Red O staining and Triglyceride GPO-POD enzymatic detection.3.To study the effects of hydroxysafflor yellow A on the activity of fatty acid synthase and hormone sensitive lipase by real time PCR and Dual luciferase Reporter Assay system.Results:1.Hydroxysafflor yellow A could enhance or inhibit the proliferation of 3T3-L1 preadipocytes.During 24 to 96 hours,0.01,0.1,1,10mg/L hydroxysafflor yellow A could significantly inhibit the proliferation of 3T3-L1 preadipocytes.And 100 mg/L hydroxysafflor yellow A could only inhibit the proliferation in the 24-hour action time significantly.After 120 hours,hydroxysafflor yellow A could stimulate the growth of 3T3-L1 preadipocytes in a dose-dependent manner.2.Hydroxysafflor yellow A could stimulate or repress the differentiation of 3T3-L1 preadipocytes.0.01,1 mg/L hydroxysafflor yellow A decreased the lipid content in the differentiation day 4,8 and 12,while increased it in day 16.100 mg/L hydroxysafflor yellow A mainly stimulated the lipid formation in the day 4 and 16 during the differentiation of 3T3-L1 preadipocytes.3.Hydroxysafflor yellow A enhanced the activity of fatty acid synthase and hormone sensitive lipase during the differentiation of 3T3-L1 preadipocytes.Conclusions1.Hydroxysafflor yellow A could enhance or inhibit the proliferation of 3T3-L1 preadipocytes in specific proliferation stages with specific concentration。2.Hydroxysafflor yellow A could stimulate or repress the differentiation of 3T3-L1 preadipocytes in specific stages with specific concentration.3.Hydroxysafflor yellow A enhanced the activity of fatty acid synthase and hormone sensitive lipase during the differentiation of 3T3-L1 preadipocytes.The stronger stimulation effect of hormone sensitive lipase than fatty acid synthase may the main cause lead to the reduction of lipid formation during differentiation of 3T3-L1 preadipocytes.