Effect Of Immunity-enginery Acupuncture On Big Rat Of Lasting Exquisite Sport And Study Of Its Function-mechanism

Objective:To study the influence that the Acupuncture pay on the mouse of long-term acute sport,and probe into the mechanism of Acupuncture nerve-internal secretion-immunity network(HPA).Methods:59 Clean 8-week-old healthy male SD rats,according to numbers,were randomly divided into four groups:control group(n=10),high-intensity swimming training group(n=17),the group of high-intensity swimming training with Moxibustion and Acupuncture(n=16),the group of high-intensity swimming training with moxibustion and electro-Acupuncture(n=16).Long-term(8 weeks) high-intensity (150min) swimming training exercise-induced immunosuppression to establish the rat model,select the three points of Guanyuan,Baihui,Zusanli,put simple moxibustionon three points of Guanyuan,Baihui,Zusanli,and put electro-Acupuncture on the point of Zusanli while put Moxibustion on the points of Guanyuan,Baihui,and then observe Acupuncture intervention on long-term high-intensity exercise stress state of the immune function of rats.From the way of nerve-endocrine-immune network,probe into the mechanism of Acupuncture intervention.Measured by radioimmunoassay using rat hypothalamic ACTH,CRH,CORT and IL-2 concentration;the use of flow cytometry determination of rat T-lymphocyte subsets CD3~+,CD4~+,CD8~+,CD161~+(NK cells) number; use ELISA determination of serum SIL-2R,γ-IFN concentration;the use of 3H-tdr infiltration method of testing blood lymphocyte mitogen in vitro proliferation of PHA.Results:Comparing to the control group,the IL-2,γ-IFN of the high-intensity training group decreased significantly((P＜0.01,P＜0.05),NK cells are on a downward trend(P＞0.05), which imply that the eight-weeks high-intensity swimming training resulted in rats'IL-2, IFN-NKC immunoregulatory network disorder occur;Although the IL-2,γ-IFN,NK cells of the group of high-intensity training with Moxibustion are lower than that of the control group,but IL-2 is not statistically significant(P＞0.05).Compared to the group of the high-intensity training,the values of theγ-IFN,IL-2 of the group of high-intensity training with moxibustionare still higher than that of high-intensity training group;Although the IL-2,γ-IFN,NK cells of the group of high-intensity training with electro-Acupuncture and moxibustion also decline,but it is not of statistical significance(P＞0.05).Comparing to control group,itsγ-IFN in the value still significantly higher than that of the high-intensity training group(P＜0.05).Comparing each other,theγ-IFN,NK cells of the group of high-intensity training with Acupuncture and moxibustion+ are less than that of the high-intensity training with electro-Acupuncture and moxibustion,but no statistical significance(P＞0.05).Comparing to the control group,the weighs of the rats of the group of the high-intensity training decreased significantly(P＜0.01),the SIL-2R is significantly higher(P＜0.05), and the weighs of the rats of the group of the high-intensity training with electro-Acupuncture and Moxibustion are significantly lower than the control group (P＜0.01),but still higher than that of high-intensity training group(P＞0.05),the SIL-2R of the two groups with Acupuncture is not only significantly lower than that of the high-intensity training group(P＜0.01),but were significantly lower than that of the control group(P＜0.01)。Comparing to the control group,CD3~+,the ratio of CD4~+/CD8~+,and the NK cells of the high-intensity training group decrease significantly(P＜0.05),while CD4~+ increase slightly,but CD8~+ also increased,so the ratio of CD4~+/CD8~+ still downward trend(P＞0.05);CD3~+,CD4~+,the ratio of CD4~+/CD8~+ of the group of high-intensity training with Moxibustion increased in both,in which CD4~+ with significance(P＜0.05),CD3~+ is very significantly higher than that of high-intensity training group(P＜0.01),but NK cells of the group of high-intensity training with Moxibustion decrease significantly(P＜0.05);The T-lymphocyte subsets of the indicators except the NK cells of the group of high-intensity training with lectro-Acupuncture and Moxibustion decreased slightly(P＞0.05),the remaining increase in both,including CD3~+,CD4~+ with a very significant significance (P＜0.01,P＜0.01),the indexes of the T-lymphocyte subsets of the group of high-intensity training with electro-Acupuncture and Moxibustion are higher than that of high-intensity training group,CD3+,CD4+ are of significance(P＜0.01,P＜0.05).Comparing to the control group,the spleen index,thymus index,the proliferation ability of lymphocytes of the high-intensity training group were significantly reduced(P＜0.01, P＜0.01,P＜0.05),and the three indexes of the group of the high-intensity training group with Moxibustion and electro-Acupuncture and the group of high-intensity training with Moxibustion are on a downward trend though,but no statistical significance(P＞0.05),in the numerical,the spleen index and thymus index of the two groups with Acupuncture are significantly higher than that of high-intensity training group(P＜0.01).Comparing to the control group,the CRH,ACTH,CORT of the high-intensity training group increased all,in which ACTH,CORT are significant(P＜0.01,P＜0.05),and the CRH,CORT of the group of high-intensity training with Moxibustion has been a downward trend,but no significant(P＞0.05),despite the ACTH of the group of high-intensity training with Moxibustion significantly higher than that of the control group (P＜0.01),but comparing to the group of high-intensity training the CRH,ACTH,CORT reduced all,in which ACTH,CORT is very significant(P＜0.01) statistically;Compared to the control group,CRH,ACTH of the group of high-intensity training with electro-Acupuncture and Moxibustion slightly increased,and CORT is downward trend, but they are not significant(P＞0.05),compared to the group of high-intensity training group,the CRH,ACTH and CORT of the group of high-intensity training with electro-Acupuncture and Moxibustion reduce all,in which ACTH,CORT are significant (P＜0.01) statistically.Comparing each other amongst Acupuncture groups,the CRH, CORT of the group of high-intensity training with electro-Acupuncture and Moxibustion are slightly higher than that of the group of high-intensity training with moxibustion,but it is not significant statistically(P＞0.05),the ACTH of the group of high-intensity training with Moxibustion and electro-Acupuncture are significantly lower than that of the group of high-intensity training with moxibustion(P＜0.05).1.After high-intensity swimming stress of eight weeks,mind,appearance,body weight of rats are deteriorated,the rats' blood immune indexes:IL-2,γ-IFN,NKC,CD3~+,CD4~+,ratio of CD4~+/CD8~+ of T-lymphocyte subsets,and the spleen index,thymus index,blood lymphocytes to mitogen in vitro proliferation of PHA showed a significant decrease,while the immunosuppressive factor SIL-2R concentration,HPA axis in CRH,ACTH,CORT were significantly increased.2.Simply putting moxibustionon Baihui,Guanyuan,Zusanli not only can improve the percentage of CD3~+,and can significantly increase the percentage of CD4~+ of T helper cells,reduced concentration of CD8~+ of suppressive T cells,caused the ratio of CD4~+/CD8~+ increased,to some extent can prevent the decline of proliferative response of thymus index,spleen index and blood lymphocytes to PHA mitogen under the state of long-term high-intensity exercise stress,but decline of NK cells' quantity lack of positive regulatory role under the state of long-term high-intensity exercise stress.Moxibustionplay limited regulatory role on IL-2-IFN-NKC immunoregulatory network,it can still prevent to some extent the decline of IL-2.3.Electro-Acupuncture on the points of Bilateral Zusanli and Moxibustion on the points of Guanyuan and Baihui can not only effectively reduced the decrease in NK cells,increased the ratio of CD4~+/CD8~+,but also promote CD3~+,CD4~+ with a significant role,can also effectively prevent the decline vitro proliferative response of thymus index,spleen index and blood lymphocytes to PHA mitogen.o rats under the state of long-term high-intensity exercise stress.They can play a more comprehensive positive role on adjusting IL-2-IFN-NKC immunoregulatory network than simply Moxibustion under the state of long-term high-intensity exercise stress,which can effectively prevent decline in the number of IL-2,γ-IFN and NK cells of the rats.4 Whether simple Moxibustion or electro-Acupuncture combined with Moxibustioncan regulate the hormone of the HPA axis from the three levels,improve the function of HPA axis to avoid HPA axis function sustained over-hyper,through reducing ACTH,CORT and CRH content.It can achieve a benign intervention role disorders of the neuroendocrinef induced by long-term strenuous exercise stress.Conclusions:1 Long-term high-intensity exercise stress can not only lead to the nerve appearance, body weight of rats deteriorated,and can cause shrinkage of the structure of immune organs of rats,the functional degradation,reduction in the number of immune cells,inhibit secretion of immune molecules,IL-2-IFN-NKC immunoregulatory network disorder,show that exercise induced rats immunosuppression.And long-term high-intensity exercise stress cause the continued excessive HPA axis hyperfunction,resulting in excessive synthesis of CORT rats,and then resulting in neuroendocrine-immune dysfunction,which may be the main reasons of leading to emergence of immune function suppression(exercise-induced immune suppression)2 Acupuncture can reduce the long-term strenuous exercise stress on the immune function of the damage,and can effectively prevent exercise-induced immunosuppression,its mechanism may be:on the one hand,correcting dysfunction of the HPA axis,and then down the concentration of CORT to achieve the protective effect of immune function of the body of long-term high-intensity exercise stress state,and effectively prevent the movement of the occurrence of immunosuppression;on the other hand,related to directly adjustment disorder of IL-2-IFN-NKC immunoregulatory network and then positive regulation of closely linked immunodeficiency.3 the regulation that Acupuncture pay on HPA axis function disorder of rat of long-term high-intensity exercise stress of rats is multi-channel and multi-level,different methods of Acupuncture intervention play a positive role differently,the results of this study show: Acupuncture combined with Moxibustion pay more comprehensive adjustment on the immune function of rats than the simple Moxibustion.