| 0bjective We definituded the time point of forming posterior capsule opacification (PCO) using animal model in rabbits and then studied the mechanism of molecule about PCO and ERK signal pathway.Methods We observed the complexion of PCO in every time point using microscope after the rabbits were undergone ECLE on the right eyes,and measured the PCNA of posterior capsule by immunohistochemical method, to definitude the time point of the earlyest and the markedest of PCO; To measure Cx43 by immunofluorescence method,to measure typeâ…£collagen by immunohistochemical method,to measure MMP-2, TIMP-2,FAK,ERK1/2 at the posterior capsule both surgery eye and comparison eye by westorn blot and FQ-PCR method in hereinbefore two time point.Group A is the earlyest of PCO,group B is the markedest of PCO,group C is comparison eye.Results The formation of PCO was discovered after the operation at the 1st month by cranny light microscope,the markedness at the 3th month;PCNA at the posterior capsule was expressed after the operation at the 2nd week, the markedness at the 3th month; There were not Typeâ…£collagen,Cx43, MMPs-2,TIMPs-2,FAK, ERK1/2 at the posterior capsule in group C ,but there were Typeâ…£collagen,Cx43, MMPs-2, TIMPs-2, FAK, ERK1/2 at the posterior capsule in group A,B. There was remarkable difference between group A and group B.Conclusions A model of PCO in rabbits can be successfully established by using ECLE; The formation of PCO was comfirmed after the operation at the 2nd week,the markedness at the 3th month;ERK signal tramsmit pathway played a key role in the process that LECs adjusted and controled ECM composing:the rudimental LECs engendered exceptional transference due to enabled ERK1/2,the expression of MMPs and TIMPs were adjusted and controled by MARK signal pathway,so as to the exudation of typeâ…£collagen increased in the ECM,the attaching and multiplication ability of LECs were swelled,promoting the happen and development of PCO.
|
PDF Full Text Request