Effect Of Aerobic Exercise On Splenic Immune Function And Its Autonomic Nerve Function

Purpose:Taking autonomic nerves system of spleen as main line of research, frome three aspects(superior center,inferior center and organ) to study the effect of aerobic exercise on some kinds of neurotransmitter in autonomic nerve system,and combined the changes of immunological function of spleen to analyze the relation of immune and autonomic nerve system.Accordingly,from the aspect of autonomic nerve system to study the mechanism of aerobic exercise increasing immunological fucinon,wich will richen body building theory of aerobic exercise.Methods:With the healthy male SD rat as the subject,the experiment divides the SD rats into exercise group(swim) and control group(quiet).The exercise group swim free from load(6 times/week,1～2h/time,the endurance incease 0.5h by each 2 weeks,8 weeks in all).The experiment analyses the esterase volume of Catecholamines and Acetylcholine in blood,nervous tissue and spleen tissue by way of Chromatography With Electrochemical Detection,detects the volume of aminophenol nervoustransmmiter in nervous tissue by way of automatic amino acid analyzer,and reflects the immunity function of spleen by using the spleen index,the cabability of T lymphopoiesis of spleen,the sub-T lymphocyte of blood.So it can look into the inner mechanism of lasting aerobic exercise to spleen immunity function.With healthy women of 61～70 years old as the subjects,the experiment divides them into aerobic exercise group and lasting-sit group by whether have the habit of aerobic exercise.By contrasting the difference of the blood routine,the antibody in blood,the sub-T lymphocyte of blood,the Catecholamines and Acetylcholine in blood,and Heart Rate Variability,the experiment analyses the influence lasting aerobic exercise makes to spleen immunity function and the autonomic nervous regulative function,and analyses the relationship between them,then it can provide further experimental proof for looking into aerobic exercise improving the autonomic nervous mechanism of body immunity function. Results:After the experiment,by contrast with the control group of rat,the proliferative cabability of spleen T lymphosyte is improved,the rate of blood CD4+ and CD4+/CD8+ are increased in exercise group,which means 8 weeks' moderate intensity swim can improve the spleen-mediated immunity function.The volume of stimulant amino acid in the hypothalamic tuberal part of rat is dcreased,the volume of rejective amimo acid increased,the volume of Catecholamines drceased,the volume of the stimulant amino acid in spinal cord intermediolateralis,the rate of stimulant amino acid and rejective amimo acid,and the volume of Catechomamones dcreased,the volume of NE in spleen tissue dcreased,all of which means the irritability of sympathetic nervous circulus in spleen is restrained.By contrast with the women in lasting-sit group,it is found that the women in aerobic exercise group have the lower rate of peripheral blood CD8+ and T lymphosyte and higher rate of CD4+/CD8+.The difference of Heart Rate Variability in both group is obvious.It is concluded the whole heart rate variation dgree of old women is increased,the turgor variation of pneumogastric nerve vagus may be increased,and the conformation between sympathetic nerve and pneumogastric nerve vagus is inclined to pneumogastric nerve vagus.There is close relationship between the body immunity function and autonomic nervous function.Conclusion:The lasting aerobic exercise can improve the immunity function of spleen,the mechanism may be related to the restraint that the irritability of the spleen sympathetic nerve is got under the stress after lasting aerobic exercise.This kind of restraint can be happened in both nerver center and peripheral region of spleen autonomic nervous regulative circulus,and also have relationship with aminonergic nerve,catechonergic nerve and cholinonergic nerve.