Effects Of Xiaotan Tongluo Gel On Bone Metabolism And TRPV1 Expression In A Rat Bone Cancer Pain Model

Objective: To observe the analgesic effects and mechanisms of Xiaotan Tongluo Gel (XTTL Gel消痰通络凝胶)on cancer pain by evaluating the mechanical allodynia threhold , body weight, markers of bone metabolism in serum and TRPV1 expression in a rat model of bone cancer pain (BCP). Methods: A rat model of BCP was established, the rats were applied with placebo gel and XTTL gel, observing the behavior of the rats, detecting ICTP , BAP and TRPV1 expression of the rats by methods of molecular biology as immunohistochemistry, ELISA, real-time PCR and Western blot. Results: The mechanical allodynia threshold of rats applied with XTTL Gel was significantly higher than that of model group(p<0.05),the ICTP in serum of rats with XTTL Gel was much lower than that of model group(p<0.05),and TRPV1 mRNA and protein expression in the bone and hypothalamus of the rats given XTTL Gel were lower than that of model group(p<0.05). Conclusion: XTTL Gel may alleviate bone cancer pain by inhibition of bone resorption and down regulation TRPV1 expression in the bone and hypothalamus .