The Innate Immunical Effect Of Toll-like-receptor 9 In Nasal Polyps Pathogen Mechanism

Purposes:. To understand the ultrastructure feature of human nasal polyps, sinusitis and inferior turbinate tissues and cells , and the expression of osinophil cationic protein (ECP) in these tissues.Methods: 5 cases of CRSwNP,5cases of CRS and 5 cases of in-ferior turbinate mucosa undergoing sinus surgery were gathered from Xiehe hospital afflilting Huazhong Techenical University ,to detect the expression of ECP by site hybridization,celeberate the primary human nasal mucosa epithelial cells cultures ,and find the morphology of these tissues and cells through the light and/or electric microscopy examina-tions.Results:1. under rhe light and/or electric microscopye the eosinop-hilic cells have highter in CRSwNP group ,degranulating and cytolytic or legs.2.the level of ECP in CRSwNP group and in CRS group were higher than in inferipor turbinate group,they have difference among them,but in CRSwNP group and in CRS group ,there were no difference between them.3.The methods of primary cell culture of human nasal mucosa were proved to gain pured epithelial cells(90.1%).Conclusion: The finding showed that the ultrastructures have chan-ged and ECP expressed higher in CRSwNP and in CRS. Purpose: By determininge the presence and function of TLR-9 protein in primary epithelial cell cultures of chronic rhinosinusitis with nasal polyps(CRSwNP),and try to explain the effection of innate immune recognition playing in the pathogenesis of CRSwNP.Methods: Fetching 5 inferior turbinate and 5 CRSwNP mucosa undergoing sinus surgery were established their primary epithelial cell cultures,flow cytometry was used to confirm purity of the cell and to assess expression of TLR-9 protein,western-blot was used to measure the TLR-9 protein.Results: Data showed that TLR-9 protein were showed both in inferior turbinate and CRSwNPgroup primary epithelial cells,but the level of expression in CRSwNP group was lower.Conclusion: The finding of TLR-9 protein is both expressed in normal and diseased sinonasal epithelial cells and the expression is decreased in CRSwNP suggest that impaired innate immune responses to pathogens via TLR-9 on sinonasal epithelial cells, eosinophilic infiltrate,mucosal higher reactions . Objective : To investigate the regulatory effects of Toll like receptor ( TLR9) ligands unmethylated CpGmotifs(CpGDNA) which effect human sinonnasal mucosa epithelial cells and observed the expressions of TLR9 and ECP protin in the epithelial cells by primary culture.,and try to find that the innate immune system via TLR9 play ports in the human nasal polyps mechanism.Methods : Putting 0μg/mL,5μg/mL CpG DNA in the cell culture plates with human nasal mucosa epithelial cells ,after 6 hours,Western-blot method was used to examine the effects of CpG DNA on expressions of protin for TLR9 and ECP ,and compaired the relationship of them.Rusults : 1.CpGDNA was up- regulated the expression of TLR9 ;2. The level of TLR9 was higher and the level of ECP was lower after situmul-ation by the ligand of CpGDNA,3. The relationship between the TLR9 and ECP was still uncer-tainly,it need more spieces and data to indentified.Conclusion :These findings may suggest that innate immune responses to pathogens via TLR9 represent a mechanism in chronic inflammatory sinus disease,and CpGDNA was up- regulated the expression of TLR9.