The MicroRNA Analysis Of Fibrous Dysplasia

1. Background:Fibrous dysplasia can affect many bone in your body. Most people with the disorder have only one affected bone—a form called monostotic fibrous dysplasia—and develop no signs or symptoms. When the condition affects more than one bone, it's known as polyostotic fibrous dysplasia. Fibrous dysplasia may cause few or no signs and symptoms, particularly if the condition is mild. Signs and symptoms may develop during childhood, adolescence or adulthood. If you have the polyostotic form, you're more likely to develop signs and symptoms, usually by age 10. More severe fibrous dysplasia may cause: Bone pain. Difficulty walking, Bone deformities, Fractures.MicroRNAs (miRNAs) are endogenously expressed non-coding RNAs of 21-25 nucleotides, which post-transcriptionally regulate gene expression in plants and animals. Recently it has been recognized that miRNAs comprise one of the abundant gene families in multicellular species, and their regulatory functions in various biological processes are widely spread. There has been a surge in the research activities in this field in the past few years. From the very beginning, computational methods have been utilized as indispensable tools, and many discoveries have been obtained through combination of experimental and computational approaches. In this review, both biological and computational aspects of miRNA will be discussed. A brief history of the discovery of miRNA and discussion of microarray applications in miRNA research are also included.2. Methods and results:Four patients and two normal control were enrolled in our study. After RNA was distilled by TRI_ZOL, microRNA hybridization was carried out. The chips were scanned with LuxScan 10K/A dual channels laser scanner of CapitalBio Company to look for the information of differently expressed genes.By contrast with normal control, we find that the expression of 22 microRNAs were enhanced and he expression of other 17 microRNAs were declined. In these microRNAs, the most prominence microRNAs changed in our results were mir-34a/b and mir-133a/b. Via pictar assay, we find that the target genes of mir-34a/b were related to major gene which influenced development and proliferation of bone. The target gene of mir-133a/b was related to muscle maturation.In addition, the change of mir-181b and mir-20b were two important microRNAs in our results. Based on our analysis and previous reports, mir-181b and mir-20b may be related to the expression of VEGF. So we investiged the expression of VEGF in patients tissue and serum by western blot, RT-PCR and ELISA.The results of western blot, RT-PCR and ELISA showed that the expression of VEGF in patients was markedly increased. This result could explain the phenomenon that patients with Fibrous dysplasia have the tendency of haemorrhage in the course of operation.