Apoptosis And Intervention Study Of Brain Injury Of Rats Induced By Lipopolysaccharide

PartⅠ:Establishment of infectious brain injury model of rats induced by LPSObjective:To explore a new kind of methods of establishing brain injury model of rats induced by LPS—injecting LPS through external carotid.Methods:160 rats were injected LPS or NS respectively and decapitated at different time points to take brain tissue samples for use.Results:Significant change were observed in LPS groups,the levels of Brain EB content,NSE and GFAP protein were all higher in LPS groups at different time points comparing with those in corresponding NS subgroups(P＜0.01).Conclusions:By injecting LPS into infant rats' external carotid,the infectious brain injury model could be established steadily and reliably.PartⅡ:Expression of AIF and the apoptosis of nervous cells on infectious brain injury model of rats induced by LPSObjective:To study the Expression Of AIF and the apoptosis of nervous cells on infectious brain injury model of rats induced by LPS.Methods:the expressing levels of AIF protein were detected by immunohistochemistry technology and the apoptosis of neural cell was measured by TUNEL technology after the injury brain model were established.Results:The expression levels of AIF protein and the apoptosis of neural cells in LPS group were all higher than that of NS groups(P＜0.05).Conclusion:Up-regulating expression of AIF protein and the apoptosis of neural cell indicated that apoptosis might play important roles in brain metabolic homeostasis associated with the pathophysiology of brain injury induced by LPS injection.PartⅢ:Protection of HGF in the infectious brain injury model of rats induced by LPSObjective:To investigate the Protection of HGF in the infectious brain injury model of rats induced by LPS.Methods:The infectious brain injury model were established by giving LPS to the rats,at the same time HGF was given by the same methods to observe the expressing of GFAP,NSE,AIF protein,EB content and the apoptosis number of neuron to study the action of HGF in such model.Results:The levels of NSE,AIF protein,EB content and the apoptosis number of neuron were all lower in HGF groups at different time points comparing with those in corresponding LPS subgroups(P＜0.01).Conclusion:The earlier use of HGF in brain injury induced by LPS could lighten the damage of brain.PartⅣ:Protection of MT in the infectious brain Injury model of rats induced by LPSObjective:To investigate the Protection of MT in the infectious brain injury model of rats induced by LPS.Methods:The infectious brain injury model were established by giving LPS to the rats.At the same time MT was given as the same as the method of HGF to observe the expression of GFAP,NSE,AIF protein,EB content and the apoptosis number of neuron to study the action of MT in such model.Results:In MT protective groups,Compared with LPS groups,EB content and the expression levels of NSE,GFAP, AIF protein and the apoptosis nerve cells were lower,the difference was significant(P＜0.05).But no significant difference was observed between MT advanced groups and MT 0 hour groups(P＞0.05).Conclusions:MT maybe have the protective effect to the infectious injury brain,.The possible mechanism is that MT can inhibit the expression of AIF which could be related to apoptosis of nerve ceils.