Study On The Association Of Ureaplasma Urealyticum And Mycoplasma Genitalium With Mucopurulent Cervicitis In Women

ChapterⅠ.Associations of Ureaplasma urealyticum and Mycoplasma genitalium with Mucopurulent cervicitis in WomenObjective To study the association of U.urealyticum and M.genitalium among women with nongonococcal,nonchlamydial mucopurulent cervicitis in women.Methods The study population consisted of two different groups including STD clinic female patients with nongonococcal,nonchlamydial mucopurulent cervicitis and healthy women.Cervical samples were collected from all subjects.The specimens were tested for the presence of U.urealyticum by culture and M.genitalium by polymerase chain reaction,respectively.Information about socio-demographic characteristics,medical history,sexual behaviour was gathered through a questionnaire.Results The rate of positive culture for U.urealyticum was 81.42% (184/226) in patiens with mucpurulent cervicitis,while 64.41%(76/118 ) in heathy women.The rate of positive culture with Uu≥10~4 ccu/mL was significanthy higher in patients with mucpurulent cervicitis(42.48%),than that in control group(16.95%) (χ~2=22.61,P＜0.001).The prevalence of M.genitaliurn was 11.06%(25/226) in patiens with mucpurulent cervicitis,while 0.85%(1/118) in heathy women.There was a significant difference between the two groups(χ~2=11.58,P＜0.001).By multivariate regression analysis,M.genitalium was strongly associated with multiple sex partners,mucopurulent secretion and≥10 polymorphonuclear leukocytes per high-power field on Gram stained cervical smear(P=0.001,P=0.042 and P=0.027).Conclusion This study indicates that infection with U.urealyticum at a high concentration may be related to mucopurulent cervicitis,and M.genitalium is associated with nongonococcal,nonchlamydial mucopurulent cervicitis among female patients attending STD clinic. ChapterⅡ.Association of Ureaplasma urealyticum biovar and genotype with Mucopurulent cervicitisObjective To study the association of U.urealyticum biovar and genotype with nongonococcal,nonchlamydial mucopurulent cervicitis.Methods The study population consisted of two different groups including STD clinic female patients with nongonococcal,nonchlamydial mucopurulent cervicitis and healthy women(as controls).U.urealyticum biovar and genotype were differentiated from U. urealyticum positive cultures by polymerase chain reaction and single-strand conformation polymorphism analysis.Results The rate of mba genotype 3/14 from biovars 1 was the highest in both groups,30.98%(57/184) in patients with mucopurulent cervicitis and 43.42%(33/76) in controls.The rate of genotype 2B from biovars 2 was 16.30%(30/184) in patient group,while 6.58%(5/76) in controls,and there was a significant difference between the two groups(χ~2 = 4.367,P=0.037). Genotype 1,3/14 and 6 from biovar 1 was dominant in healthy population(81.58%).Conclusion This study indicates that genotype 2B of U.urealyticum biovars 2 may be associated with nongonococcal,nonchlamydial mucopurulent cervicitis among female patients attending STD clinic.