Compound Gc Extract Antiarrhythmic Pharmacodynamics And Mechanism

Objective: Cardiac arrhythmia is serious symptom in the heart diseases and one of the main dead reasons.In order to study GC compound, we did research on the antiarrhythmic action and the mechanism.Methods: To investigate the protective effects of GC compound on experimental arrhythmia, arrhythmic animal models were established by different drugs. The preventive effects of ventricular fibrillation produced by chloroform inhalation in mice were observed. Through the arrhythmic rats&mice models established by aconitine, the antiarrhythmic effects of GC compound were evaluated. Making the arrhythmia rat models with alcium chloride, barium chloride and pituitrin respectively.The effects on arrhythmic model of Guinea pig produced by strophantin K were studied. The evaluating indicators were latent time & duration of cardiac arrhythmia. We investigated the effects of GC compound on arrhythmic rats established by adrenaline and acetylcholine (Ach) respectively to clarify the mechanism through adjusting the function of heart sympathetic nerve & pneumogastric nerve. Lipid peroxidation (LPO) content was measured with thiobarbituric acid chromatometry and the method of pyrograllol autoxidation was used to determine superoxide dismutase (SOD) .We examined the content of nitric oxide (NO) and the activity of nitric oxide synthase (NOS) in serum and cardiac muscle. Through these researches, the function of GC compound on the anoxemia of cardiac muscle and free radical were investigated. All data were processed by SPSS13. 0 for Windows and presentation of experiment results in (x|-)±s.Results: The effects of GC compound on five different experimental models of arrhythmia are obvious and concentration dependent, except calcium chloride and barium chloride.It can increase the activities of SOD and NOS, improve the NO concentration and decrease LPO level.Conclusion : It has been discovered in experimental researches that GC compound has antiarrhythmia effects which displays certain dosage-effect relationship. Its mechanism may be associated with adjusting the function of heart sympathetic nerve & pneumogastric nerve, eliminating free radical, dilating blood vessel and improving microcirculation.