| Objective:To investigate expression of transforming growth factor beta on "setting broken bone mixture" of invigorator the circulation of blood and benefit kidney during the repairing defect with autologous spongy bone.Methods:90 adult male or female rabbites do not tell from adult male or female rabbites,weigh from 2.0kg to 2.5kg.90 adult male or female rabbits were randomly divided into five groups.Every group is eighteen rabbits,nine rabbits are test groups,nine rabbits are control groups,The experimental group feed with "setting broken bone mixture" and the control group feed without Chinese drug.The test groups and control groups rabbits(with barbital sodium 20mg/kg intravenous anesthesia) pick up radius middle part of left and right front feet of rabbits to full exposing under without bacterium to cut skin,subcutaneous tissue, separating front tendor and periosterm.20mm standard bone defect modal was made with bone grinding machine.taking spongy bone of bilaterally ilium making 1-2mm particle with ronger or scissor,repairing left and right radical bone defect with above the bone spongy particle.The experimental group feed with "setting broken bone mixture" and control group feed without "setting broken bone mixture".The rabbites inject cefazolin sodium by 0.1g/kg four days after operation in order to prevent wound infection.Then Sacrificed at 1,2,3,4,6,8 weeks after surgery to draw materials for histological observation,immunity chemical staining of TGF-β,in situ of hybridization of their cDNA probes,electron microscope andRT--PCR for observation and analyzing the Expression of transforming growth factor beta of invigorator the circulation of blood and benefit kidney on "setting broken bone mixture"(nature copper xuduan gusuibu ground beetle) during the repair bone defect with autologous spongy bone.Results: Histological observation The experiment group have part implant bone necrosis,part implant bone cells concentrating,even vanish,there is inflammation in haematoma with fibers cells bone cortex grow into the implant bone with smaller cartilage and blood vessel from two weeks to four weeks,There are vary blood vessel in implant bone with net new bone and turning into marrow cavity from four weeks to eight weeks,The control group The bone defect have vary inflammation with part bone cells vanish on one week in the control group,there is a lot of fiber cells in bone defect one two weeks,there are smaller new bone on four weeks,The bone cells is up very much from six weeks to eight weeks The electron microscope observation:the test group and the control group find part trabeculae of destroy bone,some sphacelus with a little inflammatory cells in the one week,the cell like fusiform and there some collagen fibers with nucleocytoplasmic concentration at the around of cells in the two weeks,but the control group is not obvious fusiform cell and a little collagen fibers with nucleocytoplasmic concentration in the cell around,there are more capillary in the experiment group than the control group and some forming bone lacanaes,bone canalicalies in the four weeks,however there a little capillary and forming some bone matrix,the test group bone matrix and bone corpuscle chromocenter is obvious,the endoplasmic reticulum become more in the cell,There are some trabecaulace of bono and bone canaliculi above the bone cells in the control group,forming bone cells and woven bone is obvious in the test group at the eight weeks,but there little ossification and woven bone in the control group than in the test group.Immunity chemical staining observation:Two groups are inflammation reaction when test group and control after operation for one week.at the same time TGF-βis expression of negative reaction. Expression of TGF-βis expression of weak positive when they were operated for two weeks,it is expression of stronger positive when they were operated for four weeks, it is expression of positive when they were operated for 6,8 weeks.However, TGF-βof expression is expression of negative reaction in the control groups when they were operated for one weeks and it was positive when they were operated for 4 weeks and it is weaker expression when operation for 2,6,8 weeks.Situ hybridization: TGF-βof the experimintal group and control group are weak positive after one week;it is stronger positive after four weeks in test groups;it is positive expression after 6,8 weeks.However,The control groups is positive after 4 week and weaker positive after 2,6,8 weeks.RT-PCR observation:relative expression of TGF-βis3.69±0.01 in the test group in the one week,relative expression of TGF-βis1.00±0.00 in the control group in the one week;relative expression of TGF-βis 2.35±0.02 in the test group in the two weeks,relative expression of TGF-βis 1.40±0.01 in the control group in the two weeks;relative expression of TGF-βis 3.18±0.01 in the test group in the four weeks,relative expression of TGF-βis 1.59 in the control group in the four weeks,relative expression of TGF-βis 2.67±0.02 in the test group in the six week,relative expression of TGF-βis 1.44±0.01 in the control group in the six week;relative expression of TGF-βis 2.59±0.01 in the test group in the eight week, relative expression of TGF-βis 1.27 0.03in the control group in the eight week Conclusion:"setting broken bone mixture" may be passing through add up quantity of TGF-βand shorten repairing time in repairing defect with autologous spongy bone and give some theory reliance to clinic...
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