| Objective:To explore the mechanism of Jiedu Decoction for preventing and treating Acute Drug-induced Liver Damage(DILD) caused by Tripterygium Glycosides(TG).Methods:In the first experiment, to explore the dosage of TG for building the proper model of acute DILD, 50 normal KD mice were divided into 5 groups, and different doses of TG tablets were given to 4 groups respectively, leave l group as normal samples. The dosage of TG for the proper model of acute DILD is recorded.In the second experiment, 90 new mice were divided into 6 groups in average: one as normal group, one as model group, one was given Tiopronin, and the others were given low, middle and high dose of Jiedu Decoction respectively. 7 days later, all the groups, except the normal one, were given TG tablets according to the dosage recorded in the first step. 24 hours later, all the mice were anatomized for observing. The effects of Jiedu decoction on ALT, AST, TNFα, MDA, SOD, GSH , LPO, Bcl-2 and CYP2E1 were recorded and analyzed.Results:In the first experiment, the acute liver damage of the mice with 20 times dosage of TG for adult is obvious, and the death rate of them is low. So, 20 times of TG for adult is the proper dosage for building the model of acute DILD. In the second experiment, compared with the normal group, all the above indexes for model group are abnormal (P<0.05); however, the indexes for all the mice in the treated groups are improved than the ones for the model group; and the indexes for the group with middle dose of Jiedu Decoction are improved obviously (P<0.05).Conclusion:Jiedu Decoction is an effective drug to prevent and treat acute DILD, which mechanisms includes: lessening the degeneration and promoting the cytothesis of hepatic cells, elevating the antioxygen ability of hepatic cells, decreasing the active expression of CYP2E1, lessening inflammatory cell to infiltrate, reducing the release of cytokine especial TNFαand suppressing apoptosis of hepatic cell.
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