The Research On The Action And Mechanism Of Ginseng Meliorating IR

Insulin resistance(IR)means the target organs or the organizations are insensitive to the insulin,thus it decreases the biological effect of the latter and leads to a series of changes in pathology and physical function.In recent years,the cases of diabetes,hypertension, obesity,disorder of blood fat,arteriosclerosis are increased every year since the living style and dietary structure have been changed,and IR is assumed as the foundation and correlation of these diseases.So IR becomes a focus in the scientific research field.There is no medicine has been recognized as an ideal one to cure IR by most people,although researchers have studied it deeply.While herbs can cure diseases with its characteristic of various approach and target point,so they have the advantages in treating some metabolic diseases.And it has been proved from clinic by doctors of Chinese Medicine with the method of treatment based on symptoms under the guidance of holism.But the metabolism of action is retarded,for most cases lack of experimental data for complementary proof. Although American Ginseng also has been proved effective in curing IR by my tutor in clinic,there is an undiscovered metabolism to be found,and the intention of this research project is just to try to find out it and affirm the foundation of the action.This research project covered two sides of content,one is on document research,and the other is on experimental research.After retrieved about 500 pieces of documents pertaining to IR on the internet,we comprehend the pathogenesis,the method of treatment,and medicine or herbs commonly used on the whole,During the search course,we also learn how to establish and evaluate the IR model in rats,and get a deep comprehension in lipocyte cytokine,such as tumor necrosis factor-α(TNF-α),leptin,adiponectin(APN)and resistin etc,as well as the new research trends about them.On the basis of above research,we carry out the experimental research.First,we set up the IR model in rats by feeding them high fat diet combining injecting small dose of STZ once to them.Secondly,we use different elution fraction group from American Ginseng to treat the IR rats compared with rosiglitazone.Thirdly,we observe the difference within each group on the side of glycometabolism,lipometabolism,insulin sensitivity,lipocyte cytokine level,mRNA of glucose transporter-4(GLUT-4)in skeletal muscle and resistin in white fat tissue.The results of the search are that all groups of 30 percent alcohol elution fraction,60 percent alcohol elution fraction,distilled water elution fraction and rosiglitazone can decrease FBG and FINS in blood serum,and have an obvious difference with the model group.On lipometabolism aspect,all treatment groups have the effects of reducing TG,TC and FFA in blood serum.From the results we get as mentioned above,we further discuss the action metabolism.We use method of radioimmunoassay and enzyme linked immunosorbent assay to measure TNF-α,Leptin,APN and Resistin in serum separately.As to mRNA of GLUT-4 in skeletal muscle and resistin in white fat tissue,we use RT-PCR method.From the data we obtain, we may tell American Ginseng can ameliorate IR from both lipocyte cytokine angle and gene expression angle.At last,we draw the conclusions as follows from the research project.The first one is the IR model in rats were successfully made by high fat diet combined with injecting small dose STZ.The second one is that American Ginseng has the good effects on glycometabolism and lipometabolism,and the active site is 30 percent alcohol elution fraction,60 percent alcohol elution fraction.The third one is American Ginseng is available of ameliorating IR by affecting TNF-α,Leptin,APN and Resistin.The last one is that American Ginseng can also ameliorate IR by boosting up mRNA expression of GLUT-4 and lowering mRNA expression.