Study Of Neuroendocrine And Immunization About Post-TBI

Part 1:Study on neuroendocrine changing of post-TBIObjective:To observe the change of the patient's neuroendocrine hormone and inflammative marker in serum after medium and severe TBI.Methods:48 patients were selected from the traumatic center of our hospital and their GCS were all under 12(without endocrine disease).They were phlebotomized 1d 3d 7d 14d 21d after injury and we preserved their serum in refrigerator under 80 degrees below zero after centrifugalization.PGN,COR,T3,T4, E2,TES,EPO,FSH,GH,INS,LH,PRL,TSH,IL-2R,IL-6were detected by the automate chemiluminescence machine.According to the reference ranges,the indexes detected at different times can be classified and with calculation we got the rate which may be higher,lower than the normal level or in the reference ranges demonstrated by the diagram.We calculated the data of every group with median using statistic methods and compared with diagrams.Results:In the 15 kinds of endocrine- and immune- indexes,at least one index was abnormal at one time point of all the patients.We demonstrated this consequence with table5-38.The rate of the change which occured at the male patient's endocrine hormones and immune functions after medium and severe TBI was high.The levels of T3,T4 mostly decreased at first,then restored to the normal level;LH may be high or low,mostly showed to increase;PRL and E2 demonstrated to increase;PGN increased only for a short time;INS had no obvious change;GH and EPO increased obviously;COR mainly increased,but at a few circumstance it may decreased;TES decreased obviously;IL-2R and IL-6 increased obviously.Conclusions:The rate of the neuroendocrine and immune changes which occurred after TBI is high and it will accelerate the recovery of the patients after taking the related therapies. Part 2:A Study of the relationship between immunosuppression and antibrain-antibody(ABAb)after traumatic brain injuryObjective:To establish an animal model to prove the production of ABAb after TBI;also mild hypothermia therapy and immunosuppression therapy can decrease the ABAb.Methods:18 adult New Zealand white rabbits(no matter male or female)were divided into 3 groups,every group had 6 rabbits,all 3 groups were trephined of 7mm diameter cranial hole and fluid percussion applied.Group A—mild hypothermia therapy group;Group B—immunosuppression therapy group;Group C—control group,treated with nothing after fluid percussion,fed normally.All three groups of rabbits were phlebotomized 4ml blood through the ear fringe vein 0d,3d,7d,14d,21d,28d after injury.The blood serum must be centrifuged and then conserved with -20℃.These specimens were tested by ELISA,observed the dynamic change of Antibrain-antibody(ABAb).Except that,2ml blood phlebotomized through the ear fringe vein must be anticoagulated in order to counting the number of the white blood cell.Results:A low level of ABAb had been found in the blood serum of all the rabbits before bitted;After LFPI,every group had its respective peak value of the ABAb.The peak value of group A,B appeared later and lower than group C.There was statistically significant difference between group A and C,group B and C(P＜0.05).After LFPI,the number of the WBC of groups A and B was fewer than group C which showed that after mild hypothermia and imrnunosuppression therapy,the immune function of groups A,B had been decreased.There was statistically significant difference between A and C group,B and C group(P＜0.05).Conclusions:1.In nature state,there was a low level of ABAb in the blood serum of all the rabbits;2.After traumatic brain injury,the concentration of ABAb was higher than nature state;3.After traumatic brain injury,the increased ABAb would likely to attack self brain tissues,so that we considered that traumatic brain injury could have the characters just like autoimmune diseases;4.After traumatic brain injury,mild hypothermia and immunosuppression therapies could decrease the concentration of ABAb by repressing the immune functions;5.The experiment proved that immunosuppression therapy will become a new methode dealling with traumatic brain injury.