Study On Correlation Between Osteopontin And Craniopharyngioma Prognosis

[Objective]:In the present study,we investigated the changes in the expression of OPN mRNA and its main receptor integrin alpha v beta 3(αvβ3)in craniopharyngioma,and further discussed the relationship between this factor and craniopharyngioma recurrence as well as the growth velocity after relapse,aiming to provide an effect way to predict the recurrence of craniopharyngioma and the condition of infiltrative growth.[Methods]:We all collected 65 operation examples and clinical data from the craniopharyngioma patients since Nov.2005 to Jul 2007 Of the 65 examples,there are 32 Adamantine Epithelioma(AE)examples and 33 Squamous Papillary Tumor (SP).Follow-up visit to Mar 2008,we begin to prospective cohort study.To detect OPN mRNA of craniopharyngioma and the expression of OPN and integrinαvβ3,we use the techniques of fluorescence quantitative polymerase chain reaction(FQ-PCR) and immunohistochemical S-P method.Moreover,Cavalieri principle was adopted to calculate the volume and growth velocity of the 36 recurrence examples following operation in this study.[Results]:Of the 32 AE examples and 33 SP examlpes,there were 11 and 3 examples relapsed respectively.They all had statistical significance(P＜0.05)of relapse rate between AE and SP groups and OPN mRNA as well as its diffrential expression of protein.Furthermore,there was an significant difference(P＜0.05)of growth velocity between AE and SP types in the 36 recurrence examples following operation,but there was an apparent negative correlation(P＜0.05)shown in the Ct values. Comparetively,there was a significant difference in the expression of integrinαvβ3 between AE and SP groups,and there was shown an obviously positive correlation in the expression between OPN and integrinαvβ3 in craniopharyngioma.[Conclusion]:1.There is an expression of OPN in craniopharyngioma;2.The relationship between the expression of OPN mRNA and craniopharyngioma relapse as well as the growth velocity after relapse is showed an positive correlation;3.Integrinαvβ3 exression may play an important role in craniopharyngioma and its prognosis as well as recurrence.4.OPN promoting the craniopharyngioma relapses and invasive growth by integrinαvβ3,might be predict the risk of craniopharyngioma relapse.