Mechanism Study On Acupuncture's Effection On MRNA Expression Of CLUT4 And Akt2 In Insulin Signal Trandsduction Pathway Of Experimental Insulin Resistance Rats

Implication of insulin rasistance(IR) leads to a series of changes on pathology physiology,which are significant risk factors to many disease included in stroke. And curative effects of acupuncture on stroke ,diabetes adipositas and so on which connected with IR have been generally accepted.Objective:To develop a rat model of IR,and to study the effect of acupuncture on expression of GLUT4 (glucose transporter 4) and Akt2 (protein kinase Bp) in somatic muscles of IR rats and the mechanism of acupuncture therapy in IR,which can prevent and cure stroke and other disease correlated with IR.Method: 1 .Male Wistar rats were were randomly divded into 4 groups: The IR model rats without treatment group(M group), the IR rats with head acupuncture treatment group(HA group), the IR rats with back Shu-acupoint acupuncture treatment group(SA group) and the normal controls group(N group) and each group has 8 rats. The rats of M,HA and SA groups were given the high fat diets for 4 weeks to be IR model rats. Then the HA group were treated with cluster acupuncture on scalp points and the SA group were treated with Pishu Shenshu and Yishu acupoints electroacupuncture for 2 weeks.2.The blood samples were collected to evaluate FINS,FBG,TC and TG, to calculate ISI ( insulin sensitivity index) and measure BW to identify the IR of model rats and observe the curative effect of acupuncture. 3 . The protein expression of GLUT4 and Akt2 in rats skeletal muscle were observed by immunohistochemistry. 4.The GLUT4 and Akt2 mRNA of skeletal muscle was determined with RT-PCR(reverse transcription-polymerase chain raction).Results: 1. Compared with N group,the BW,FINS and TG in M group were significantly increased, and ISI was decreased(P<0.05).Compared with M group,the BW,FINS in HA group and SA group was significantly decreased, and ISI was increased (P<0.05), withnot statistical significance between HA group and SA group (P>0.05).There was no statistical significance of the increase of FBG and TC in M group compared with N group, as well as the decrease of BW and TG in HA group and SA group compared with M group(P>0.05).2. The result of immunohistochemistry was shown the protein expression of GLUT4 and Akt2 in M group rats skeletal muscle was weaker than that in N group. And there was stronger expression in HA group and SA group than that in M group without significant defference between acupuncture groups.3. The result of RT-PCR was shown the expression of GLUT4 mRNA in M group rats skeletal muscle was significantly decreased,compared with N group (P<0.05) .And compared with M group, it was increased in HA group and SA group(P<0.05) without significant defference between them(P>0.05).The expression of Akt2 mRNA in M group rats skeletal muscle was significantly decreased,compared with N group (P<0.01) .And compared with M group, it was increased in HA group and SA group(P<0.01) without significant defference between them(P>0.05).Conclusions: 1 .IR can be ameliorated by acupuncture without significant defference between HA group and SA group,which can prevent and cure stroke and other disease correlated with IR. 2. The genetic transcription of GLUT4 and the ability of conveying and utilizing glycose in IR rats skeletal muscle cells can be enhanced by acupuncture, which is one of the amelioration mechanisms of acupuncture.3. Acupuncture may enhance the Akt2 expression and raise the amount of Akt2 mRNA in IR rats , which enhances the transposition of GLUT4 in the PI3-K/Akt signaling pathway, contributing to the main mechanismof improved post-receptor IR and decreased hyperinsulinemia in IR rats,which also is one of the amelioration mechanisms of acupuncture.