Effect Of Transforming Heme Oxygenase-1 Gene On Discordant Cardiac Xenograft

Stable cervical cardiac xenotransplantation model of guinea pigs to SD rat was successfully established by Heron's cuff vessel anastomosis technique. The usage of purified Chinese cobra venom factor administration showed excellent effects to exhaust complement C3 in rat recipient,can prevent discordant cardiac xenografts from hyperacute rejection(HAR) and establish the acute xenograft vascular rejection(AVR) model.On the base of the (AVR) model,Intracoronary perfusion of recombinant adenovirus vector ex vivo mediated heme oxygenase-1 gene transfer to guinea pig donor hearts during cold preservation after harvest,the method were efficient and effectively.The successful transcription of exogenous heme oxygenase-1 gene in the xenografts was confirmed. Extensive expression of exogenous gene in perivascular myocardial cell and coronary capillaries attenuated inflammatory cell infilitration in the xenografts, relieved impairment of immune reaction and significantly prolonged xenograft survival time. Gene transfer that can induce localized expression of exogenous gene and preserve the endothelial cell would be a new strategy for overcoming AVR.