IL-6 Expression Of Hypothalamus And C-Jun N-terminal Kinase (JNK) Signaling Pathway Changes Of Gastric Mucosa In The Rat With Stress Ulcer Caused By Fluid Percussion Brain Injury

Objective:Through the establishment of stress ulcer model of the rats induced by traumatic brain injury in the study,we want to know:(1) the relationship between gastric mucosal blood flow,gastric juice PH value and gastric mucosal injury index;(2)the expression of Central interleukin-6 in the rat hypothalamus stress,as well as the mechanism in stress ulceration;(3) C-Jun N-terminal kinase(JNK) signal pathway is or not involved in the activation of stress ulcer formation,as well as its pathway inhibitors can or can't reduce gastric mucosal injury.Methods:stress ulcer model is established by fluid percussing injury.20 male SD rats were randomly divided into control group,one hour group,six hours group and 12 hours group after injuried(n=5).In each group of rats gastric PH value,gastric mucosal blood flow,gastric mucosal injury index are measured,and observed the histology of gastric mucosa.The expression and distribution of IL-6 in hypothalamus and p-JNK in gastric mucosal are detected by Immunohistochemistry and Westernblot.We establish a inhibitor group in order to know whether inhibitors can or not reduce gastric mucosal injury by the observation of PH value,gastric mucosal blood flow,and the histology.Results:(1) One hour after injury,we found the injury in the gastric mucosa.And the condition of injury became worse as time passed.The UI was increased.One hour after the injury there was a temporary increase in blood flow.It's the peak.Then the blood flow was declined and keeped stabilizing.But the blood flow was still lower than the blood flow of control group.One hour after the injury the PH value was rapidly declined.The the lowest point was at 6h.Then it was increased.But the PH value of experimental groups was obviously lower than the PH value of control group.They had no negative correlations between PH value of gastric juice and gastric mucosal injury.But the PH value of experimental groups was obviously lower.It shows potential negative correlations between the gastric mucosal blood flow and gastric mucosa damage.(2)IL-6 immunoreactive cells widely distributed in nerve cells of hypothalamus in the stressed rat,especially in the paraventricular nucleus.(3) We found in the epithelial cells of ulcer injury p-JNK was strongly positive response by Immunohistochemistry.c-Jun N-terminal kinase(JNK) signal pathway inhibitor SP600125 can significantly reduce gastric mucosal injury.Conclusion:(1) stress ulcer model is established by fluid percussing injury.(2) They had no negative correlations between PH value of gastric juice and gastric mucosal injury.But the PH value of experimental groups was obviously lower than the PH value of control group.It shows potential negative correlations between the gastric mucosal blood flow and gastric mucosa damage.(3) The expression of IL-6 near the hypothalamic paraventricular nucleus (PVN) was obvious,which may be through the paraventricular nucleus(PVN) mediated by the central cholinergic neurons excited,HPA axis,as well as the activation of 5-HT system metabolism increased stress involved in the process of stress ulceration in some pathophysiological mechanism.(4) The c-Jun N-terminal kinase(JNK) signal pathway is activated in Stress ulcer and participated in the occurrence of stress ulcer.(5) JNK signaling pathway inhibitors SP600125 reduce gastric mucosa damage of the rat in stress ulcer.As an effective means,it probably provide us a new idea of the treatment of stress ulcer.It maybe provide us very important guidelines for a new treatment strategy.