| Current cariology indicates that dental biofilm is an initiation factorof caries. Recently, natural products have attracted increased interest asan alternative choice in caries prevention. There are many theories andprescribes in ancient Chinese natural medicine dealing with dental caries,which facilitate the study of preventing dental caries by herbal medicine.Our study group has screened the active traditional Chinese medicineand active compounds to provide empirical drug of study of cariostaticmechanism in future. Magnolia offinalis was shown to inhibit thegrowth of cariogenic bacteria and GTFs. However, the systematic studyof Magnolia offinalis in caries prevention has not been carried out andits molecular mechanism of anticaries activity has never explored tillnow. The aim of this study is to investigate the chemical components ofMagnolia offinalis and their anticaries effect, to trace out and identifythe most effective component and to explore its pharmacologicalanticaries mechanism.Methods: The chemical components of Magnolia offinalis were extracted and further fractioned. A series of in vitro anticariousexperiments, the inhibition of growth, acid production, glucangeneration, have been carried out. The inhibition effect of activecompounds of Magnolia offinalis on oral bacterial biofilm has beenevaluated by determination of MBEC, MBIC and MBRC. CLSM wasused to observe morphosis and activity of treated or untreated oralbacterial biofilm. The pH cycling model was used to evaluate the effectof Magnolia offinalis on enamel demineralization.Results: Component A, B, C, D were extracted from Magnoliaoffinalis. Component B was identified as the effective section andproved to be same effective as magnolol and honokiol in vitro anticariesexperiments. Single-bacterial biofilm was readily formed onMBECTM-Device. The values of MBEC, MBIC and MBRC indicatedthat magnolol and honokiol were effective in inhibiting the biofilmformation and reducing the 1 day developed biofilm. The effect ofmagnolol and honokiol on oral bacterial biofilm not only inhibitsbacteria biofilm growth, but also adjusts biofilm structure andcomposition. Magnolol and honokiol had no effect on enameldemineralization.Conclusion: Component B were successfully extracted fromMagnolia offinalis and proved to be the effective section. Magnolol andhonokiol had significant anticaries effect in vitro and in biofilm. Weinvestigated the effect of magnolol and honokiol on bacteria and toothderemineralization by pharmacodynamic experiments for the purpose of defining their anticaries activity mechanism. |
