Xiaxi Oral Liquid Shr Catecholamines, Adhesion Molecules, And Ras Impact Experiments

Essential hypertension is a disease which represented mainly by the rising blood pressure of systemic circulation, it can cause grave damage to heart, brain, kidney and other important viscera. This essay systemic study the treatment of essential hypertension with xiaxi oral liquid from reference review, theory study and experimental study. This prescription is established by the principle of modulating liver and kidney, and putting forward that superficial vessel's damnification by toxin is the crux of essential hypertension. It has innovation in Hypertension Therapy. Through the experimental study of Chinese medicine's effect of reducing pressure as ACEI and ARB, superficial vessel's damnification by toxin basis of pathology is explained by study of the impact of the Chinese medicine to cell adhesion molecules. this has never been reported before, so this essay is practical and innovative.1 Reference reviewThis essay systematically summarizes the treatment of essential hypertension with TCM as follows: the etiology and pathogenesis, syndrome differentiation with differentiation of symptoms and signs, Single Chinese Medical Herb, Traditional Chinese Patent Medicines , different treatment with differentiation of symptoms and signs. Some problems such as syndrome differentiation, criterion for the evaluation of therapeutic effect and scientific research were reviewed and prospected.This essay also summarized the experimental study results and advances on the principle of preventing and treating in EH according to the dialectic, pathologenesis, treatment, experimental study and advances.2 Theory studyBy discussing the name, localization,pathogenicfactors and pathogenesis, the important point of knowing poison and the relationship between poison and superficial venules and lymph vessels of EH with Chinese medicine theories quoting from classics works and combining modern research results,we put forward the theory that the main mechanism of EH is the maladjustment of liver and kidney and damnification of superficial venules and lymph vessels,and the valid path of treat EH is the way of adjustment of liver and kidney and removing toxicity and obstruction.3 Experimental study3.1 The antihypertension and cardiovascular protective effect of Xiaxi oral liquid in SHR40 12-week-old male SHR were divided randomly into blank control group ,captopril control group,Irbesartanl control group ,and Xiaxi oral liquid control group and 10 wister-kyoto rats were set as normal control(WKY group).The various markets were observed.The results showed that Xiaxi oral liquid obviously improve the SHR's behavior and general condition and had a certain effect on lowered blood pressure and extremely significant statistic sense after dministration 8 weeks(P<0.01). Xiaxi oral liquid can reduce the ratio of LVW to BW by 24.05%(P<0.01). It can also alleviate the hypertrophy of cadiocyte, protecte blood vessel endothelium and reduce the proliferation of media vascular smooth muscle cells.The result showed that Xiaxi oral liquid has the function of lowering blood pressure and protecting to cardiovascular system in SHR.3.2 The experimental study of effect of Xiaxi oral liquid on SHR serum catecholamine (CA)Studying the density of CA in serum of SHR with Enzyme-Linked Immunosorbnent Assay (ELISA) .The results showed that the density of serum CA in SHR is obviously higher than that in WKY rats(P<0.01). Xiaxi oral liquid can reduce the level of serum CA in SHR ,which had a significant statistic sense in comparing with blank control group after administration 20 days (P<0.05),and can reduce the level to normal standard after 40 days (P<0.01) , the tendency lasting for 60 days. The result showed that Xiaxi oral liquid has the function of reducing the density of CA of serum of SHR,and indicate that the increasing catecholamine standard may be the inner basis for excitement,hyperactive liver-yang ascending and hypertension in SHR. Xiaxi oral liquid can depress the activity of sympathetic nerve, lower blood pressure and improve behavior and general condition through lowering the density of serum CA in SHR.3.3 The experimental study of the influence of Xiaxi oral liquid on solubol intercellular adhesion molecule-1 (sICAM-1) density in plasma and intercellular adhesion molecule-1 (ICAM-1) expression in kidney arteriole in SHRStudying the density of sICAM-1 in plasma with ELISA and ICAM-1 expression in kidney arteriole with immunohistochemistery(IMH) . The results showed that Xiaxi oral liquid can reduce sICAM-1 density in plasma and ICAM-1 expression in kidney arteriole in SHR. The results revealed that the injury of endothelial cell induced by higher expression of ICAM-1may be one of the pathology basis about damnification of superficial venules and lymph vessels by poison.And Xiaxi oral liquid can alleviate the injury of endothelial cell through reduce the expression of ICAM-1 in SHR so that educe the function of removing toxicity and obstruction.3.4 The study of effects of Xiaxi oral liquid on recirculating and local ennin-angiotensin system(RAS) in SHRDetecting the plasma renin activity(PRA) and plasma angiotensinâ…¡(Angâ…¡) content with radio-immunity, serum Angiotensin-Converting enzyme (ACE)activity with spectrophotometer, angiotensinâ…¡receptor type 1(AT1R) expression in abdominal aorta with immunohistochemistery(ICH) and the gene expressive content of AT1R in thoracic aorta with Reverse Transcription -polymerasechain reaction(RT-PCR) in rats. The results showed that Xiaxi oral liquid can reduce PRA , Angâ…¡content, ACE activity, AT1R expression in abdominal aorta and the gene expressive content of AT1R in thoracic aorta.It revealed that Xiaxi oral liquid has the function of lower blood pressure as Angiotensin-Converting enzyme inhibitor(ACEI) and angiotensin receptor block(ARB).