Application Of Proteomics To Find Clues As Well As Screening Of Pancreatic Cancer Early Diagnosis Of Signs And Pathological Mechanisms, The Cells Of The Study Fragile X Mental Retardation Protein (fmrp) Interacting Molecular Target

Upon the coming of post-genomics era, the life science will from the study of gene switch to explore the function of gene. Protein is the product of gene and performs several of functions in different processing of life. The expression processing of whole proteins in cell is dynamic and characterized by spatio-temporal specificity. On the other hand, it is evident that the functional evolution of proteins in higher eukaryotes results more from combinatorial diversification of regulatory networks than a substantial increase in gene number. Based on all of the above facts, proteomics is proposed, which is defined that the study of all the protein forms expressed within an organism as a function of time, age, state, external factors, etc. Proteomics is a new approach that integrates some key fundamental technologies, such as high-throughput protein separation and profiling, mass spectrometry, genomics and proteomics database, and browser-based bioinformatics tools to extract information from these databases. There are two different but complement strategy for applied proteomics: one is expression proteomics, the other is functional proteomics. Hence, understanding the protein-protein interaction and the connectivity of protein with cellular signaling network certainly will benefit from the advantage of proteomincs.This work applies proteomics into the basic and clinic research, which is divided into two following parts: Proteomics of Human Pancreatic Adenocarcinoma and The Study of Proteins Interacted with FMRP by Proteomics1 Proteomics of Human Pancreatic AdenocarcinomaThe large majority (about 90%) of pancreatic carcinomas (PCs) are believed to originate from the ductal system, with the remaining ten percent originating from acinar and other cells. PC is the fourth cause of cancer death in Europe and the USA. Patients usually have advanced disease at the time of clinical presentation. Over half of them already suffer from metastases, and the 5-year survival rate is less than 2%. This highlights the need for an early diagnosis that would increase the chance of survival by allowing treatment at more favorable stages. Unfortunately, despite many years of intensive research, we are still lacking an early and reliable method for detection of PC in asymptomatic patients. Efficient early cancer detection requires knowledge of histology and molecular genetics of precursor lesions combined with development of sensitive and specific screening tests. Only in recent years, the study of the mRNA profiles and the protein composition in cancer and normal tissue has become feasible. For pathologists, it is a powerful molecular scanner that gives comprehensive insights into the protein composition of the normal and cancerous tissues they examine. The application of proteomics provides major opportunities to understand disease mechanisms and to identify cancer-specific proteins...