Tissue Engineering Arteries Organizations To Build A Preliminary Experimental Study

[Objective]: 1) Obtain the smooth muscle cells(SMCs) of canine' s blood vessels by cell culture technique to provide cell resources for the experimental study on artery tissue engineering.2) Evaluate the effect of bFGF on SMC proliferation and determine the appropriate dosage of bFGF to promote SMC proliferation on scaffold.3) Evaluate the cytotoxicity and biocompatibility of several new biodegradable materials and discuss the feasibility of these new materials as scaffolds for vascular tissue engineering.4) Carry out a primary study of artery tissue construction and provide a fundamental basis for tissue engineered artery and its clinical application.[Methods]: 1) SMCs are isolated from canine blood vessels by tissue plant method. The cells were subcultured. Observing the shape, growth of cells, the immunohistochemistry staining, and electron microscopy identified the SMCs. The purity of primary culture, the characteristics of morphology and proliferation of SMCs were observed.2) The proliferation rates of SMCs under different dosage of bFGF were estimated by MTT method. The proliferation rate of SMCs on scaffold using the dose of 3ng/mL bFGF was also estimated.3) By growth inhibition study of L929 cell strain, the cytotoxicity of several new biodegradable materials were estimated. The contact angle was assayed for comparing the hydrophilicity of several biomaterials. The RGR of SMCs cultured on different biodegradable scaffolds was also assayed and compared by MTT method. The proliferation of SMCs on scaffolds was observed by FITC and electron microscopy.4) Collagen scaffolds were seeded with ECs after 13 days of SMCs seeding and culturing. Within 2 days, a confluent monolayer of ECs was observed on surface of multilayer of SMCs.