People Calpain1, The Large Subunit Of The Role Of Protein Screening Cardiac Myocyte Hypertrophy Model Induced In Angll Function Initial Exploration

Objective: Calpain, a cytosolic cysteine protease, requires calcium ions for activity. It has been reported that calpain is involved in the degradation of myofibrillar and neurofilament proteins, and the activation of phosphorylase b kinase and protein kinase C. More recently, calpain was shown to participate in apoptosis. In order to understand the calpain-related signal transduction pathway and its changes during hypertrophy, and especially in hypertension, we screened a human heart cDNA library to find proteins that interact with calpain.Methods and results: (1) Using PCR we amplified the full-length, domain â…¡, domain â…¢ and domain â…£ cDNA of calpain (calcium-activated neutral protease, CANP) I large subunit respectively. (2) Then the fragments were cloned into pGBKT7 vector, resulting in 4 bait expression constructs (pGBKT7-CANP, pGBKT7-CANPII, pGBKT7-CANPIII and pGBKT7-CANPIV).