Mycosis Fungoides Of Telomerase And P53 And Cell Proliferation Mechanisms

ObjectMycosis fungoids (MF) is the most common cutaneous T cell lymphoma whose pathogenesis is still unclear. Recently, the infinite proliferation of tumor cell is found to be associated with telomerase. Telomerase plays an important role in tumorigenesis. In addition, P53 gene mutation can result in the development of tumor and may be associated with telomerase. There is not report about the relationship of telomerase, P53 and cell proliferation until now, so we carry out this study to explore the pathogenesis of MF.Material and methodThere were 60 biopsy specimens including 13 tumor stage of MF, 14 plaque stage of MF, 8 patch stage of MF, 13 lichen planus and 12 normal skin in this study.(1) To detect the expression of PCNA and P53 protein with Immunochemistry.(2) To detect the telomerase activity with polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) and polyacrylamide gel electrophoresis.ResultP53 protein was positive in 61.5% tumor stage of MF, 21.4% plaque stage of MF and 0% patch stage of MF. But...