Miscellaneous Dog Capillary Leakage Model The Dynamics Of The Different Capacity Of The Liquid

Part 1: Establishment of capillary leak model in mongrel dogsObjectives: Early goal-directed therapy provides significant benefits with respect to outcome in patents with severe sepsis and septic shock. The first goal of resuscitation for septic patients is recovery of blood volume. But the type of fluid to choose is still controversial. We aimed at establishing capillary leak syndrome model in mongrel dogs, and unveiling changes in blood volume and hemodynamics.Methods: A dose of 20μg·kg^-1·h^-1 E coli endotoxin 111: B4 were infused continuously for 30 min in 5 mongrel dogs. Transcapillary escape rate of plasma protein were analyzed before and after the LPS infusion to confirm the establishment of capillary leak by measuring the absorbance-decay-curve of Evans blue. Serial plasma volume and hemodynamic changes were also recorded.Results: A dose of 20μg·kg^-1·h^-1 E coli endotoxin 111: B4 infused continuously for 30 min in 5 mongrel dogs could result in elevated transcapillary escape rates (from 9.1 ±0.3%/h at baseline tol2.9 ± 0.5%/h 30min after LPS infusion, P<0.05), which means the establishment of capillary leak syndrome model. At the same time, plasma volume (34.4± 1.3ml/kg at baseline to 22.5 ± 0.5ml/kg 30min after LPS infusion, P<0.05) and mean arterial pressure were reduced(119 ± 5mmHg vs. 64±3mmHg, P<0.01). And the reduced plasma volume could be maintained for at least 3 hours. Intravenous protein pool was reduced from 2.5±0.3g/kg at baseline to 1.3±0.1g/kg(P<0.05) 30min after LPS infusion.Conclusions: Capillary leak model in mongrel dogs could be established by continuous infusion of E coli endotoxin, with dropped plasma volume and mean arterial pressure. And the reduced plasma volume could be maintained for at least 3 hours.