Experimental Study Of The Pathogenesis Of Refractory Epilepsy

Objective To investigate effect and expression of glutathione S-transferase Ï€ (GST- Ï€ ), DNA topoisomerase â…¡ (Topo â…¡ ), P-glycoprotein(P-gp), multidrug resistance-associated protein 1(MRP₁) and lung-resistance related protein(LRP) in brains from patients with medically intractable epilepsy, in order to illuminate the mechanisms underlying multidrug resistance in intractable epilepsy, and to provide new theoretical basis of diagnosis and treatment.Motheds The specimens were obtained from 32 patients with intractable epilepsy and 8 patients with arteriovenous malformations as control, immunohistochemistry for GST- Ï€ , Topo â…¡, P-gp, MRP1 and LRP.Results GST- Ï€ immunoreactivity in several capillary endothelial cells and glial cells with the morphologic appearance of astrocytes was found in normal brain samples, it significantly increased in samples from epileptic patients(t=2.47, p<0.02). Topo â…¡ immunoreactivity was not identified in normal brain samples, but it was found in 2 samples from epileptic patients(t=0.70, p>0.05). Unlike in normal brain samples, P-gp immunoreactivity was identified in several glial cells with the morphologic appearance of astrocytes and increased in capillary endothelial cells insamples from epileptic patients(t=3.75,/?