Injection Of Allogeneic Cells In The Rat Denervated Target Muscles To Promote Nerve Regeneration Research

Objective: Research the pathologic changes and mechanisms of Spinal cord anterior horn motor neurons, peripheral nerve and target muscles after peripheral nerve injury. And Study the affect to never regeneration by injecting allogenic different cells to absent-never-target muscles in rats.Methods: 36 female adult SD rats, within 120 and 150 grams, were divided into four groups randomly and averagely. Left ischiadic nerves in SD rats were cut off on an aseptic conditions, and the epineuria were operated by primary suture. Allogenic different cells were injected to rats' triceps surae muscles of four groups, which were manipulated every seven days and in all for four times after operation. One milliliter of Schwann cells with a concentration of 1×106/ml were injected to Group A. one milliliter of mixed cells with a concentration of l×106/ml, which included Schwann cells and myoblast cells as the 1:1 proportion, were injected to Group B. One milliliter of extract from the kidney endothelial cells medium were injected to Group C. And one milliliter 1 FCS-free culture medium as control group were injected to Group D. The rats were killed three months after operation, and the spinal cords of four to six segment, the left operated ischiadic nerves and the triceps surae muscles mastered were obtained for the observation of specimens on macrobody form, enzyme histochemistry and immunohistochemistry.Results: Three months after operation, the results of observation were analyzed by statistics. The macrobody form, enzyme activity and C'-Jun positive expression of treated groups were different significantly from those of control group in statistics(P<0.01).The density of regenerated neurilemma cells in unit area and the area of never Fiber in treated groups were different significantly from those of control group in statistics(P<0.01). And the quantity of motor end plate in unit target muscles, the enzyme activity and the quantity of Actin positive muscle fibers in unit area in treated groups were different significantly from those of control group in statistics(P<0.01).Conclusions: After allogenic different cells were injected to absent-never-target muscles in rats, the cells can secret many active factors continuously. The factors may be transported to soma of neurons by receptor mediated endocytosis to prevent the neurons from death and atrophy and protect Spinal cord anterior horn neurons so that the nutritional materials for never regeneration can be supplied sufficiently. Nervous active materials concentrate so high locally that it can induce regenerated axis-cylinders to grow farther and active Schwann cells to proliferate rapidly, which can promote the form of myelin sheath and the mature of never so that the function of muscle can get over as soon as possible. Moreover, not only the absent-never-target muscle can get enough nutrition in a long term, but also the degeneration of motor end plate can be stopped or postponed when the contact between absent-never-target muscles and central motor neuron is lost. In our experiment, the observational results in Group C is better than that of Group A and B. We conclude that the extract from the kidney endothelial cells medium include a lot of neurotrophic factors, so that the functions of the extract to protect the Spinal cord anterior horn neurons, promote the regeneration of never and prevent absent-never-target muscle from atrophy are superior to that of pure Schwanncells or the mixed cell. But the opponents of the extract are unkown and need to be studied. The mixed cells can be better than pure Schwann cells in functions. So we deduce that the mixed cells can co-operate to produce many other nutritive factors, which are undisclosed too.