Gm <sub> 1 </ Sub> And Methylprednisolone During Cardiopulmonary Bypass Brain Injury, And Clinical Research

Objective: To study the protective effect and its mechanism of monosialoganglioside(GM₁) and methylprednisolone(MP) on brain injuries after cardiopulmonary bypass (CPB).Method: Forty-six patients suffering from rheumatic heart valve disease were selected and divided randomly four groups: control group (n=10), GM₁ group (n=12), MP group (n=12), GM₁+MP group (n=12). Before the operation of heart, at 1 hour, 2 hours, 6 hours, 12 hours and 24 hours after CPB termination, whole blood were taken, the blood serum levels of S100B, IL-8, IL-10 and ICAM-1 were assayed by enzyme-linked immunosorbent assay(ELISA) method.Results: (1) Serum levels of S100B of GM₁ group, MP group and GM₁+MP group at each time point after CPB termination were lower than those of control group (P<0.05). (2) Serum levels of S100B of GM₁+MP group at each time point after CPB termination were lower than those of GM₁ group and MP group (P<0.05). (3) Levels of IL-8, IL-10 and ICAM-1 of GM₁ group were no difference with control group (P>0.05).(4) Levels of IL-8 and ICAM-1 of MP group and GM₁+MP group were lower than those of control group (P<0.05), Levels of IL-10 of MP group and GM₁+MP group were higher than those of control group (P<0.05). (5) Levels of IL-8 and ICAM-1 of GM₁+MP group were lower than those of GM₁ group (P<0.05), Levels of IL-10 of GM₁+MP group were higher than those of GM₁ group(P<0.05), but Levels of IL-8, IL-10 and ICAM-1 of GM₁+MP group were no difference with MP group (P>0.05).