Acupuncture On Cerebral Ischemia Reperfusion Mitochondrial Damage Factors

The brain is sensitive to ischemia. After the injury of ischemia-reperfusion, theorganism will respond initiatively or passively. it forms the complex pathologicmechanism of brain injury of ischemia-reperfusion.Because there are changes ofphase of ischemia after the brain ischemia, it provides the theoretical proof forstudying pertinent interference and improving the function of brain in the windows oftheraputic time.The recovery of mitochondria function is absolutely necessarily forcells in ischemia-reperfusion, mitochondria is rery important in series of waterfallresponses in brain injury. Around the main affairs of cell programme death conductedby mitochondria after brain injury of ischemia-reperfusion, there are series ofcomplex pathologic cascade of damage of time and space.In the brain ischemia, theoxidative-phosphorylation of mitochondria cann't process efficiently, and the creationof ATP decreased and hydrolyzation increased, the cells get function obstruction fordeficiency of energy. With the extension of the time of ischemia, the struction andfunction of mitochondria damaged, and ATP exhausted, and the function of cellsdamaged more. In reperfusion, the mitochondria manufactures many free radicles. Inthe same time, the transfer of calcium of mitochondria is inordinate, it turns topermanent penetrate, the calcium in cell plasm raises sharply. It conducts to theprogramme death in the function of correlative factors such as family of Bcl-2,enzyme of cysteine-asparagine, cytochrome-C, et.c.The brain injury ofischemia-reperfusion can induce to regeneration of nerve cells. There is endogenesicNSC activation and the function of brain can reconstruct. There is distributing andmoving regulation of regeneration of NSC. The relation between nerve-gowth-factorand nerve-special-protein and regeneration of nerve cells is close. In many cell factors,nerve-nutriton-factors is important in the regeneration of nerve. The target-inducingnerve-nutriton-factors act on corresponding nerve cells by self-secret, para-secret,transfering. The nerve cells gained enough nerve-nutrition-factors can survive, otherswill die.It is thousands of years using acupuncture to cure stroke in clinic of china. It wasrecorded in huangdineijing, jiayijing, zhenjiudaquan. For the advance of the windowsof theraputic time, the study of Acupuncture turned from sequelae and convalescencestage of ischemic stroke to acute period. It is a result of compound function of manypaths and many factors for acupuncture to treat brain injury of ischemia-reperfusion.The study of acupuncture to protect brain injury of ischemia-reperfusion is wide. Itinclude interference to damage of nerve function of rat, change ofpathologic-morphology of brain tissue, meliorate of brain metabolize and brainmicro-circle, signal-transfer of ischemic nerve cell Apoptosis. The study ofacupuncture mechanism is not only about integrated analyze of organ and system, butit is gradually broadly expand on the micro-level.the mechanism of the protectingfunction of acupuncture to brain, but it maybe is activating the potential of organism,and startup many regulatory mechanism including endogenetic anti-injury of ischemia,英文摘要 - 4 -all of them educe the protecting function to brain of anti-injury. The protectingfunction to brain maybe promote endogenesic nerv stem cells to participate in therehabilitation of fault.We made model of focal brain ischemia-reperfusion injury by middle cerebral arteryocclusion (MCAO) and simulate brain ischemia-reperfusion injury by incubatinghippocampi nerve cells culture in vitro. We carried through these reseaching work: 1.Begin with mitochondria acting a key after brain ischemia-reperfusion injury, weobserved the change of electric potential of mitochondria, the change of calciumwithin cell, and the dynamic change of MDA, GSH in brain tissue tha...