| ObjectiveInvasion and metastasis are very important features of malignant tumors, being the main factors that made patients die . Though diagnosis and treatments of tumors are being improved, the overall treatment effect is not satisfying. At present, five year survival rate of lung cancer is just 15% ; median survival time of untreated lung cancer patients is only 4-5 months, and one year survival rate is 10% [3] . The basic reason is that we are not able to control and block up the invasion and metastasis of tumor cells. With the development of molecular biology, the background of tumor has been being clearly identified. However, the molecular foundation of tumor cell's invasion and metastasis remains unknown, which makes invasion and metastasis of tumor cells become the hot and difficult questions of molecular biology on study. Nowadays, we have known that invasion and metastasis of tumor cells, just as its occurrence, involved not only the activation of oncogene, but the inactivation of suppressor gene, accompanied by the participation of cellular adhesive factors .KAI 1/CD82 is a member of transmembrane 4 superfamily ( TM4SF) , whose structure is glycoprotein of cellular membrane. In 1995, Dong et al separated a specific metastasis suppressor gene of tumor cells from chromosome 11 of human metastatic prostatic carcinoma cell line AT6. 1. This gene was named KAI1 ( named after its Chinese meaning kang ai ) gene. The metastasis suppressor function of KAI1/CD82 gene has been widely accepted . After long years study, it has been confirmed that , in most tumor cells, the metastatic cells often involves the decrease or lack of the expression of KAI1/CD82 gene1-10'11^. Integrin is the most important cellular adhesive acceptor, which has different subunit aand, and is a membrane glycoprotein . As a member of integrin family, integrin 5 is a kind of fibronectin receptor, participating in cellular signal transmission, regulating cellular adhesion and activation[13,14,15].It has been suggested that high expression ofct5 has a negative correlation with metastasis[16,17]. In spite of this, there are few foreign and domestic articles a-bout KAI1/CD82 ando5, with simple experiment and paradoxical conclusion. Recent study indicated that KAI1/CD82 and integrin can form into a complex, regulating adhesive function of integrin and influencing cell metastasis mediated by integrin [18,19,20]. So it is necessary to further realize the significance of KAI1/ CD82 ando5. By detecting the expression of KAI1/CD82 andct5 in human lung cancer, Our experiment explored the relation between KAI1/CD82 , 5 and clinical factors, analyzed the correlation between KAI1/CD82 ando5, and explained their effects on invasion and metastasis of lung caner cell.Method44 lung cancer samples, including 37 cases with nsclc and 7 cases with sclc, staged according to 1997 international lung cancer TNM staging ' were detected the expression of KAI1/CD82 ando5 by RT - PCR method and immu-nohistochemistry method. All data were analyzed using SPSSIO. 0 statistics software.ResultsAmong 44 lung cancer patients, 20 (45.5% ) showed positive expressions of KAI1/CD82 by RT - PCR , while by immunohistochemistry, 24 (54. 5% ). The results of this two kinds of experiments have statistical significance (p <0. 05). Among 44 lung cancer patients, 22(50. 0% ) showed positive expressions ofa5 by RT-PCR, while by immunohistochemistry, 27(61.4% ). The results of this two kinds of experiments also had statistical significance ( p < 0. 05 ). The expression of KAI 1/CD82 in NO stage compared with the one in Nl and N2 stage . The results of two groups have statistical significance (p<0. 05,r>0). The expression of KAI 1/CD82 in Patients of I stage compared with the ones of II , IH and IV stage. The results of two groups appeared statistical significance(p < 0. 05, r > 0). The expression of KAI1/CD82 in Patients of well differentiation compared with the cases of morderate and poor. The results showed statistical significance (p <0. 05,r >0). On the pathol...
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