Experimental Cerebral Hemorrhage Inflammatory Mechanisms Of Injury And The Intervention Study

Objective: At present, the study of cerebrovascular disease has been gene level. Appearance of a disease is not result from a single gene, the change of any physiological functions which original trigger is due to the change of gene expression. cDNA microarray was able to monitor tens of thousands of gene expression at one time, which was one of the ideal means to find gene expression of disease. This study is to detect gene differential expression in rat perihamatomal tissue (PHT) following intracerebral hemorrhage by cDNA microarray, which would be to discover the pathology mechanism of secondary damage after ICH.Methods: Wistar rats were randomly divided into sham group and ICH group(4h,ld,3d), we established the experimental ICH model by stereotaxical injecting collagenase (VII) into rats left caudate nuclei. We chose cDNA microarray of rats BiostarR-40s, and tagged sham group by cy3 fluorescence, and tagged ICH group by cy5 fluorescence, and then hybridize, inspect and analyse.Results: There are 42 differential expression genes in cDNA microarray which cy5/ cy3 ratio more than 2 or less than 0.5 at 4h after ICH, and 29 up-regulated expressed genes and 13 down-regulated expressed gene among them; There are 61 differential expression genes in cDNA microarray which cy5/ cy3 ratio more than 2 or less than 0.5 at 24h after ICH, and 35 up-regulated expressed genes and 26 down-regulated expressed genes among them; There are 207differential expression genes in cDNA microarray which cy5/ cy3 ratio more than 2 or less than 0.5 at3d after ICH, and 107 up-regulated expressed genes and 100 down-regulated expressed genes among them. These genes mostly related with stress response, immunity, inflammatory damage; cell death and apoptosis; energy metabolism; signal transduction. There are differentially expressed genes for example interleukin-lbeta(IL-B),Interferon regulatory factor l(IRF-1), S100, Vascular cell adhesion molecule 1 (VCAM-1) and so on among up-regulated genes, that indicated multi-inflammatory cytokinestake participate in pathology mechanism after ICH.Conclusion: (1) There are great number differential expression genes after ICH. (2) Genes related with inflammation injury present high expression, as early as 4h after ICH, and run through all the time (up to 3d). (3) Genes related with cell death and apoptosis markedly up-regulated expression at 3d after ICH. (4) Genes related with energy metabolism expressed lately, appeared at 24h and clearly expressed at 3d, most of them were down- regulation. (5) Gene related with signal transduction expressed quite activity.