The Angelica The Ancient Medicinal Profile And Coumarins Research

Baizhi, which was recorded in shennongbencaojing firstly, is frequently used for treating pain . Herbalogical study on history, variety, function and suitable diseases .collecting time,processing method , quality assessment and officinal part of Baizhi was carried out. We found that Hangbaizhi had the longest history and its yield field had changed. Hangzhou doesn' t produce Baizhi any more. Baizhi had the widest function in the Ming dynast, but its applied scope has become narrow more and more since the Qing dynast and some funtion such as hemastation is out of use now. It has been found that the collecting time isn' t same between the South and the North, we believe that such narration as "collected in Augest" and "collected in winter" reflected Baizhi collecting in the North after studying on documents.To fount out the producing history, production and marketing of Radix Angelica dahuricae, investigating at the spot and consulting the relating documents were carried out in Heinan ,Hebei, Zhejiang and Sichuan province. The results show that Chuanbaizhi is best in quality and largest in yield. Hangbaizhi has the longest history, but its yield is small.Eight compounds were isolated from Angelica dahurica (Fisch. ex Hoffm. ) .Benth. et Hook. f. var. formosana ( Boiss.) Shan et Yuan . Compoud I was identified as B - sitosterol , compoud II was isoimperatorin , compoud in was osthole , compoud IV was imperatorin, compoud V was xanthotoxin , compoud VI was byakangelicol .compoud VII was oxypeucedanin hydrate and compoud VI was scopoletin . Compoud I and III were extracted from Radix angelica dahuricae for the first time , while compoud V and compoud VIII were extracted from Angelica dahurica (Fisch. ex Hoffm. ) Benth. et Hook. f. var. formosana ( Boiss.) Shan et Yuan for the first time.The total content of coumarins in Baizhi was determined for the firsttime by UV. At the same time , the content of imperatorin , isoimperatorin and oxypeucedanin hydrate in different samples was carried out by HPLC .The results show that the harvest time , the processing method and the officinal position have great effect on the coumarin content. To provide scientific basis for clinical using, extraction of the total coumarin of Baizhi was carried out. Pharmacologic effect experiments have been carried out also for the first time. They show that the total coumarin of Baizhi has no distinct effect of inhibit inflammation and cann' t significantly inhibit ear swelling induced by xylene in mice. It can decrease the rats' pain caused by hot board and actic acid. The further study shows that isoimperatorin and imperatorin have the distinct effect of repress pain and are the main active ingredients of Baizhi. Pharmacology experiments show that the total coumarin of Baizhi, isoimperatorin, imperatorin and oxypeucedanin hydrate have distinct anti-spasticity effect, while byakangelicin has no effect.