Mechanism Research On Anti-hepatic Fibrosis Of Hepatic Ji Powder Based On Warm Yang And Expell Cole

Objective:To study the ancient literature of Chinese medicine with hepatic fibrosis related "Tive ji""hepatic ji"," spleen ji" theory, the principle and prescription are summarized, put forward " warm yang and expell cole" traditional Chinese medicine rehabilitation law, the Cuban side on the basis of creation of prescription" hepatic ji powder". Through the experimental study, observation of hepatic ji powder on hepatic fibrosis of rat liver cell enzymes, serum markers of hepatic fibrosis, lipid peroxidation, hepatic stellate cell and cell factors, based on warm yang and expell cole's hepatic ji powder on hepatic fibrosis mechanism.Methods:102male Wister rats were raised for1weeks, adaptability and randomly divided into6groups, each group of17. For normal control group, model group, colchicine tablets group, hepatic ji powder low dose group, hepatic ji powder dose group, high dose group of hepatic ji powder.In addition to the normal control group, other groups of intraperitoneal injection of40%carbon tetrachloride paraffin molding agent, the first0.5ml/100g body weight, after each0.3ml/100g body weight,2times a week, normal control group received the same amount of0.9%sodium chloride injection. Zhou Zao dies after4tol times a week. By8weeks, a total of12weeks, molding and by10%alcohol as the above model the only drink. Normal control group with normal saline. Model for4weeks after a random sample of each model in any ldeath, confirmed hepatic fibrosis models have been established successfully started after gavage administration in the treatment of. Specific methods are as follows:the model group and normal control group were given distilled waterl.5ml/100g body weight, colchicine tablets group according to each0.01mg/100g, hepatic ji powder low dose treatment group were treated with compound herb0.05g/200g, hepatic ji powder doses treatment group were treated with compound herb0.1g/200g, hepatic ji powder high dose therapy group was given Chinese medicine compound0.2g/200g, for8weeks.8weeks after treatment, retrobulbar blood rats were sacrificed after. Unified take rat liver right leaf specimens in10%formaldehyde fixed, embedded in paraffin sections stained with HE.Results:According to the pathological tissue section below, all of this research model of rat liver were observed in varying degrees of liver cell hydropic degeneration and fatty degeneration, fibrous connective tissue hyperplasia, infiltration of inflammatory cells, this study demonstrated successfully mould. in the model group rats liver inflammation and fibrosis degree lower than normal group were significantly higher (P<0.01); serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hyaluronic acid (HA), laminin (LN), type III procollagen (PC III), MDA (MDA), transforming growth factor beta1(TGF beta1), liver tissue connective tissue growth factor (CTGF), tissue inhibitor of metalloproteinase-1(TIMP-1), CTGFmRNA, TIMP-1mRNA, hepatic stellate cell receptors CB1and CB2, activation of hepatic stellate cells expression were higher than normal group increased (P<0.05, P<0.01); serum superoxide dismutase (SOD) compared with the normal group were significantly reduced (P<0.01).Using hepatic ji powder high, medium, low dose treatment, rat liver inflammation and fibrosis degree than those in the model group reduced significantly (P<0.05, P<0.01); serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), hyaluronic acid (HA), laminin (LN), type III procollagen (PC III), malondialdehyde (MDA), transforming growth factor beta1(TGF beta1), liver tissue connective tissue growth factor (CTGF), tissue inhibitor of metalloproteinase-1(TIMP-1), CTGFmRNA, TIMP-1mRNA, hepatic stellate cells, activation of the cannabinoid receptor CB1hepatic stellate cell expression than those in model group were significantly reduced (P<0.05, P<0.01); serum superoxide dismutase (SOD) and hepatic stellate cell receptor CB2than those in the model group increased significantly (P<0.01).Conclusion:Based on warm yang and expell cole's hepatic ji powder downregulation of hepatic fibrosis rats serum ALT, AST, HA, LN, PC III, MDA, TGF beta1, liver tissue CTGF, TIMP-1, CTGFmRNA, TIMP-1mRNA, hepatic stellate cell receptor CB1, activation of hepatic stellate cells express; elevated serum SOD and liver stellate cell receptor CB2expression may be the part of the mechanism of anti hepatic fibrosis.