| Objective:To confirm the global H3K4methylation level, and the expressive levels of the related histone H3K4-specific modifying enzymes in endometriosis.Methods:Global histone H3K4methylation level was detected with EpiQuikTM global histone H3K4methylation assay kits, and Real Time-PCR was used to detect the expressions of the histone H3K4demethylases KDM5A, KDM5B, KDM5C, KDM5D, and methyl-transferase MLL1in the eutopic and ectopic endometrium of15cases with endometriosis, which were divided into two groups:the eutopic group and the ectopic group, and also in the normal endometrial tissue of11normal cases (control group).Results:The experiment showed a global histone H3K4hypomethylation in both the eutopic group and the ectopic group, while the methylation degree was not significantly associated with the R-AFS stage. There were no statistical differences in mRNA transcripts of the gene KDM5A and KDM5D within the three groups, whereas KDM5B mRNA level was significantly increased in the ectopic group compared with the control group (p=0.001). For KDM5C, the mRNA transcripts of the ectopic group and the eutopic group were significantly increased compared with the control (p=0.037, p=0.001). As in MLL1, the mRNA levels were significantly decreased in the ectopic group and eutopic group compared with the control group (p=0.000, p=0.000). Moreover, the expressive level of gene KDM5C was negatively associated with the histone H3K4methylation level (r=-0.499, p=0.005), while the that of MLL1was positively related with the H3K4methylation level (r=0.559, p=0.000). The transcript levels of KDM5B, KDM5C and MLL1were not significantly associated with the R-AFS stage of endometriosis (p>0.05). Conclusion:The global H3K4methylation level was decreased and the expression levels of the specific modifying enzymes were aberrant in endometriosis, we may conclude that the aberrant H3K4methylation may take part in the pathogenesis of endometriosis.
|
PDF Full Text Request