The Study On Children's Serum Different Proteomics Of Henoch-schonlein Purpura With Abdominal Involvement

Henoch-schonlein purpura (HSP) is a common pediatric disease which iscaused by vascular inflammation changes. It's pathogenesis is verycomplex.Overreaction to infection, foods or drugs within human body lead toextensive permeability change of little artery and capillary associated withtissue edema and bleeding which is caused by IgA-mediated immunologicalchanges. IgA can activate complement bypass program and excessive immunecomplex result in release of vaso-active substance which lead to increasingvasopermeability and tissue edema. Eventually,exposit of immune complexwithin vascular walls or renal glomerulus membranes aggravate tissue andvascular injuries.Pathological change of HSP represent with diffuse inflammation of smallvessels including arteriola and venula besides capillaries. HSP with abdominalinvolvement is a common type of pediatric HSP and characterized by acuteand paroxysm colic abdominal pain followed by typical erythra. Because of thelate presentation of the erythra,HSP with abdominal involvement is difficult tobe diagnosed accurately and mistakes are always taken to treat it surgically justas the common acute abdominal disease.Proteomics is a advanced area of modern biotechnological techniques.Untill now,there were not reports about the serum different protein expresswithin HSP with abdominal involvement. In this study, advanced differentialin-gel electrophoresis and TOF/TOF MS are employed to explore differentprotein express within paediatric HSP with abdominal involvement andinvestigate the mechanism, diagnostic method and prognosis evaluation. Themolecular target is also studied for prevetion and medicine development. The serum samples,which are taken from children patients with HSP withabdominal involvement and acute appendicitis and normal ones,are studied forthe different protein express within paediatric HSP with abdominalinvolvement and biological index is investigated for treatment and diagnosis ofpediatric HSP with abdominal involvement.14serum High-abundance proteins are removed by Seppro IgY14SpinColumns(Sigma),analysis gel are produced with differential in-gelelectrophoresis system.The gels are analyzed with Typhoon9400fluorescentscanner and DeCyder automatic difference analysis system followed byanalysis throgh DeCyder-BVA software.55protein points are regarded assignificant points,in which protein volume arise or decrease1.5times,P<0.05,appear more than3times.55significant different express proteinpoints are analyzed with TOF/TOF-MS and MASCOT database.Amongthem,there are13differential expression proteins of HSP group and acuteappendicitis group,including3arising proteins and3decreasing proteins inHSP group,4arising proteins and4decreasing proteins in acute appendicitisgroup.Other2proteins have different expression in HSP group and in acuteappendicitis group,which need to be studied furtherly.The scores are200inaverage. It is documented by bioinformaion analysis that those proteins playroles in amino-acid biosynthesis,amino-acid biosynthesis/structural protein,translation, energy-metabolism, biosynthesis of cofactors/Isomerase/growth-factor, amino-acid biosynthesis/transcription regulationetc.Overexpression of complement C4B3is associated with complementpathway activation the same as report.α1-antitrypsin and haptoglobin are acutephase proteins and may take part in HSP's allergic reaction.Overexpression offibrinogen γ are related to fibrinolytic system activation and hypercoagulativestate in HSP. In addition there are3unnamed proteins may be associate withthe mechanism of HSP with abdominal involvement.The above proteins may become molecular markers of HSP or targeting proteins for pharmaceuticaltreatment.The levels of serum differential expression proteins's RNAexpression are studied with real-time PCR between HSP with abdominalinvolvement group and acute appendicitis group. It is shown that proteinexpression is associated with mRNA expression.In this study,the serum different protein expression in HSP withabdominal involvement is investigated with proteomics technology andoverexpression of complement C4B3,fibrinogen γ,haptoglobin,apolipoproteinE etc. May construct a new hypothesis about the mechanism of HSP withabdominal involvement,and may be taken as marker for diagnosis of HSP withabdominal involvement.The study has important significance and wideapplication prospect.Proteomics is a complex work,because of the limitedconditions,this study has some deficiency,we need work harder in order tofurther enrich and perfect the test results.