Translated By The Party-based Dose The Poisonous Medicine Dose Explore - Sini Soup On The Rat Hemorrhagic Hypotension Model Dose - Response - Poison Relationship Preliminary Research

Objective:Dosage is the soul of prescription. Dosage in Chinese herbal prescription is closely linked with effect, efficacy and safety. it is very necessary to compare the dosage, efficacy and toxicity of toxic herb based on dosage conversion of classical prescription through experimental study, explore the effective and safe dosage conversion standard of classical prescriptions in accord with clinical practice nowadays, discuss actual dosage of classical prescription of Treatise on Febrile and Miscellaneous Diseases, provid empirical evidence for clinical practice.Method:To compare the pharmacologic effects of "Sini Decoction" with dosages of different convertsion criteria. 1 Ameliorative effect of "Sini Decoction" with Different Dosages on blood pressure, heart rate, respiration in Bloodletting Hypotension RatsForty male Wistar rats were randomly divided into four groups: bloodletting hypotension group (Control group), Sini Decoction low, middle and high dosage groups(SND L group, SND M group and SND H group). The models of hypotension by bloodletting were replicated. The whole curves of blood pressure (BP), heart rate (HR), respiratory (R) were recorded during experiment by multichannel physiological signal acquiring system. At the end of the experiment the changes of curves were analyzed. 2 Ameliorative effect of "Sini Decoction" with Different Dosages on Hypoxia-Ischemia Conditions in Bloodletting Hypotension RatsForty male Wistar rats were randomly divided into four groups:bloodletting hypotension group (Control group), Sini Decoction low, middle and high dosage groups (SND L group, SND M group and SND H group). At the end of the experiment, the changes of the arterial blood-gas levels, blood biochemistry, serum lactate dehydrogenase (LDH) and lactic acid (LA) were observed, the heart and kidney tissue were taken to determine the activity of malondialdehyde (MDA) and superoxide dismutase (SOD). The lung tissue was obtained to determine the activity of nitric oxide (NO) and nitric oxidesynthase (NOS).Result: 1 To be compared with control group, systolic pressure (MSP),HR,respiratory frequency(RF) increased significantly in SND L group. MSP and HR were increased significantly in SND M group. HR was increased significantly in SND H group. There was no significant difference among groups in diastolic pressure (MDP) and mean arterial pressure (MAP). 2 To be compared with control group, the levels of PaC02 decreased significantly and the levels of PaO2 raised significantly both in SND L group and SND H group, the levels of B cBase (Ecf) and cHC03-(P) dropped in SND H group. Serum levels of LDH, LA, Cr, CK, ALT and AST reduced dramatically in SND L group. The activity of MDA decreased and the activity of SOD increased in kidneys in SND L group. The activity of MDA increased and the activity of SOD decreased in hearts in SND H group. NO content and T-NOS activity in lungs in SND L group decrease drastically.Conclusion:Sini Decoction with proper dosage appear to elevate BP, strengthening heart and promoting respiratory function, ameliorate hypoxia-ischemia status in Bloodletting Hypotension Rats, protect and maintain the function of vital organs, but no obviously enhanced effects were observed as the dosage increased. Side effect were noted when high dosage (especially Radix Aconiti Lateralis Preparata) were to be used. These results suggested that we should pay more attention to the possible risk when increasing the dosage of Sini Decoction in clinical practice. Rational Combination, well-formed formula, reasonable methods of herbal decoction and administration are keys to ensure the safety and effectiveness in TCM practice...