The Study Of The Clinical Effects And Functional Mechanisms Of Pd No.1on Parkinson Disease

Part OneThe Clinical Obersevation of PD NO.1on Parkinson DiseaseObject i veStudy the curative effect of PD NO.1to Hypokinesia Type and Rigid Type Parkinson.MethodsUnder the guide of Randomized control study method, I divided56eary-middle Hypokinesia Type and Rigid Type Parkinson patients into two groups, treatment group and control group. There was29patients in treatment group and27patients in control group respectively. We treated the first group with dopa plus PD NO.1(made up of:Ebony, Dogwood, Yam, Aconitem, Rehmanniae, Root of herbaceous peony, Licorice et al) and the second group with dopa only. We recorded the observation and improvement of the two groups and analysed the curative effects of PD NO.1.ResultsThere is no statistical significant between the curative percentage of two groups.The curative percentage of treatment group is62.69%while the corresponding number is51.85related to control group.But the improvement of treatment group is dominant when we take clinical sympotoms and UPDRSâ…¡, â…¢ estimate into consideration. It is illustrated that treatment group is better than control group at the level of recovery and the ability of daily life. We estimated the two groups with PDQ-39and got a statistical significant result. All these show that PD NO.1has great effects on improving the life quality of PD patients including movement and nonmovement aspects(constipation, dizzness and sleep).ConclusionThe clinical study demonstrated that PD NO.1has prominent effects on improving the clinical sympotoms and daily movemnt of Hypokinesia Type and Rigid Type Parkinson patients. It means that PD NO.1can improve the life quality of PD patients. Some factors such as sample population limitation and experimental period made it impossible to get a solid conclusion of PD NO.1on curing PD patients. We need more experiments and further study.Part twoThe effects of PD NO.1on Dopa level of PD Model MiceObjectiveTo see the stimulative effect of PD NO.1on nerve behavior, Dopa level and Substantia nigra cell's morphology of PD Model mice. To invistigate the mechanism of PD NO.1on PD model.Methods1. Generate PD mice modelUsing the left side of the striatum two single-injection method. Firstly, we protracted mice head three-dimensional positioning map according references:make sure the two-point coordinate of left side of the striatum. We fixed the head inject point at1.6mm of skull rostral side and2.8mm of left side of middle line. It's deapth is5. Omm. The back inject point at1.0mm of anterior fontanelle tail and4.5mm of middle line. It's deapth is5.0mm too. Secondly, we marked the coordinate of the two points. Thirdly, we digged a hole at the marked point and injiected mice with micro injection. We injiected2.5ul6-OHDA to a mice at lul/min.In order to finish the behavior detection of mice weekly after treatment, we injiected the mice with APO0.5mg/kg(prepared in sodium) to induce one-way rotation. If the mice rotated rigth more than7times one minute, we thought the model was generated sucessfully.2. TreatmentWe divided PD model mice into four groups randomly:model, PD NO.1low dose (8.558g/kg), PD NO.1high dose (17.116g/kg), and normal group. We fed mice with PD NO.1+dopa, dopa or ddH20one time/day continuously for32days. The PD NO.1high dose group was fed2times/day.3.MeasurementBehavior detection (rotation experiment), dopa level measuring, histological morphology of corpus striatum and substantia nigraResults1.The effect of PD NO.1on mice rotation performance:By comparing after or before treatment, both low and high group mice were improverd(P<0.05).The differences between low or high dose group and model group were statisticaly significant (P<0.05). It means that PD NO.1is helpful to PD mice.The difference between low and high dose group was statisticaly significant after treatment at the meanwhile.2.The effects of PD NO.1on dopa level of damaged corpus striatum:Comparing with control group, the other three groups all had low DA content in corpus striatum(P<0.05). The effect of PD NO.1on DA content was dose dependent because high dose group had higher DA level than low dose group and model group(P<0.05).3. The effect of PD NO.1on mice brain histological morphology:It was confrimed that we generated PD mice model sucessfully because of cell necrosis of substantia nigra in model, low dose and high dose groups. Mice treated with PD NO.1had more nerve cells and the number and the length of dendritic and axons were all increased daramtically. The high dose group had more dominant increase than the low dose group at the same time.ConclusionPD NO.1can improve PD modle mice in the aspect of rotation movement. It's crutive function is dose dependent. The mechanism is not very clear now. But our experiments results show that PD NO.1can promote nerve cell renew and increase secretion ability of corpus striatum in damaged substantia nigra.