Research Of The Mechanism Of Prescription Of Jianpiruangan To Restrain Hepatic Fibrosis

Hepatic Fibrosis is a chronic disease caused by the injuries of hepatic cells. It will lead to the cirrhosis, and even dead. According to the data from clinical, the percentage of cirrhosis patients in the hospital all over the country is about 14.2%, which the mortality takes the second place in the digestive system diseases. So it is worth to study how to deferred and reverse the development of hepatic fibrosis.The prescription of Jianpiruangan is a modern prescription according to the tra-ditional Chinese theory of "therapy the hepatic with spleen" combined with the di-fferentiation of symptoms and signs. In this prescription, it contains varies herbs, such as Radix Pseudostellariae, Radix Astragali, Atractylis ovata, tuckahoe, Chinese angelica root. White Paeony Root, Danshen Root, Ground Beeltle, oyster, Pangolin Scales, Bitter Orange, liquorice, hawthorn and so on. Every single herb has its own functions in the anti-hepatic fibrosis. This prescription is well used in the clinical, but the mechanism is seldom described or was studied. So we will take a deep research of this prescription to fine out how this prescription takes effects on the anti-hepatic fi-brosis.We studied this prescription in vivo an in vitro. In vitro experiments, we confirmed the therapeutic effects of this prescription and found that it could reduce the hepatic index in the hepatic fibrosis rats model induction by carbon tetrachloride, lowered the levels of ALT and AST, relieved the degrees of hepatic fibrosis. And in the next in vitro experiments, we found that this prescription could affect multifold cellular signal tunnels in the hepatic fibrosis. In the research of TGF-β/Smad tunnel, we found that this prescription could reduce the expression level of TGF-βand the numer of the TGF-βreceptors in the cells, and adjusted the proportion of the SMAD3 and SMAD7. In the research of classical WNT tunnel, we found that this prescription took effects on the anti-hepatic fibrosis was to enhanced the expression level of FRZ, but not the expressions level of WNT protein. And this prescription could reduced the expression level ofβ-catenin, but enhanced the expression of HNF-4 and HNF-6. In the research of PI3K tunnel, this prescription coudld reduce the transcription of PDGF and PI3K mRNA, raised the cAMP level in the serum, and adjusted the proportion of TIMMP-1 and MMP-3 proteins. And in the further studies, we found the rats with hepatic fibrosis got the functional turbulence in the immune system, which showed the lower proportion of the CD4/CD8 by the rats hepatic fibrosis model with spleen subordinated Resectionlie. The prescription of Jianpiruangan could reduce the secretion of IgG, and adjusted the proportion of CD subcellulars. At last, in the vivo experimental research, we found that the concentration of this prescription of 0.128mg/ml and TC10 0.025mg/ml, could enhance the number of the TRAIL receptors directly which lead the HSC-T6 cells to the apoptosis.According to the above studies, we knew that the prescription of Jianpiruangan has a good effect on the anti-hepatic fibrosis. It could affect multifold sides of the development of hepatic fibrosis, such as the cellular tunnel, adjustment the function of immune system and induction the cells to apoptosis.