| Objectives:This research is through clinical research, observation of clean wet mixture of stasis of sperm immunity against Chlamydia trachomatis infection in the clinical efficacy of infertility, a Chinese medicine treatment of Chlamydia trachomatis infection provide the basis for anti-sperm immune infertility, while others simulate the incidence of anti-sperm immune infertility way to genital chlamydial infection of rat anti-sperm immune complex model of Chlamydia trachomatis genital tract infection anti-sperm immunity rat uterus Fas/Fas-L, Bcl-2 expression of CT infection in the reproductive system of anti-sperm infertility, apoptosis of immune mechanism. And uterus and fallopian tubes by observing the morphological characteristics of the changes of chlamydia infection in rats with anti-sperm immune inflammatory changes, and stasis by giving wet mixture of traditional Chinese medicine Qing intervention of the Qingshihuayu mixtures stasis treatment of this disease effect and mechanism of action.Method:1. Clinical Research:The 40 patients met the inclusion criteria of Chlamydia trachomatis infection in anti-sperm immune infertility were randomly divided into control group, treatment group,20 patients and 20 patients in control group was treated with Western medicine alone treatment group in the conventional antibiotic therapy Add to the clear on the basis of wet circulation mixture,1 month for a course of treatment,3 patients were observed after treatment the clinical effects observed Chlamydia trachomatis cervical swab test was negative, as well as the drop of serum AsAb, follow-up Six months, the clinical pregnancy rate were compared. 2. Animal experiments:SD rats were randomly divided into 5 groups, A group (simple inflammation model+saline group), B group (treated with immune model+saline group), C group (inflammatory immune stasis model+clear wet mixture group), D group (inflammatory immune model+saline group), E group (control+saline group). Vaginal inoculation methods used were injected at different models of Chlamydia trachomatis and/or human sperm membrane antigen, simulate the natural process of pathogenesis, the establishment of animal models, and the C group were fed clean wet mixture stasis treatment. Experiment (modeling) rats before and after the weight, shape, eating activities and vaginal discharge, etc., before modeling orbital venous blood, measured levels of serum AsAb. After the experiment, orbital venous blood, measured levels of serum AsAb and drawn uterus and fallopian tubes, uterus immunohistochemical determination of tissue Fas, Fas-L and Bcl-2 protein expression, and uterine tissue and the observed morphological changes in fallopian tubesResult:1. Clinical study:overall clinical efficacy in the treatment group the cure rate was 15.00%, total effective rate was 70.00%, higher than the control group,5.00% and 40.00%(P<0.05). AsAb serum levels after treatment the treatment group dropped an average of 98.14IU/mL, the control group fell by an average 52.15IU/m, the two groups, the treatment group decreased significantly compared with the control group, the difference was statistically significant (P<0.05.) AsAb negative treatment group was 40.00%, higher than the control group,15.00%(P<0.05). CT negative treatment group was 65.00%, higher than the control group,45.00%(P<0.05). Before and after treatment, symptom scores were significantly different, indicating that treatment significantly improved symptoms. Control group, symptom scores before and after treatment shows no significant improvement after treatment, the treatment group improved significantly in patients with symptoms than the control group (P<0.05). Treated within six months of pregnancy in 2 cases, pregnancy rate was 10.00% in the control group 0 patients, no pregnancy, the two groups, the difference was not statistically significant (P>0.05), which may be related to inadequate sample size, short follow-up time related.2. Animal experiments, we found a simple genital tract inflammation, active immunization alone reproductive tract, reproductive tract inflammation +genital tract can produce active immunization group were AsAb, compared with the control group but significant differences (P<0.05), and genital tract inflammation+AsAb active immunization group than mere quantitative level of reproductive tract inflammation and active immunization model of reproductive tract alone was significantly increased, the difference was statistically significant (P<0.05). A group, C group, D group uterus Fas Fas-L and Bcl-2 protein expression and E group (control group), the difference was statistically significant (P<0.05). A, C, D in the three groups, C rats uterus Fas/Fas-L protein expression highest level, Bcl-2 protein expression in the lowest, with the D group, A group, the difference was statistically significant (P<0.05). After modeling, D rats appeared uterus and fallopian tube congestion, edema, or adhesions, to clear the wet mixture circulation after treatment (C) tissue congestion, edema, inflammatory exudate, and significantly improved adhesion, the difference was significance (P<0.05)Conclusion:1. Qingshihuayu mixtures can improve the circulation of anti-sperm immune infertility Chlamydia trachomatis (damp heat stasis type) in patients with clinical symptoms and reduce the titer of serum AsAb the same time, combined with antibiotics, a synergistic effect, improving a negative rate of CT, and shorten the course of antibiotic use and reduce the incidence of chronic inflammation, thereby increasing the chance of pregnancy in patients.2. With Chlamydia trachomatis serotype E-type vaginal inoculation of C. trachomatis infection is the more successful modeling approach, this model of Chlamydia trachomatis infection in the genital tract in an experimental platform for research work.3. Chlamydia trachomatis infection in rats with anti-sperm immune cell apoptosis barriers to the presence of inflammation, the sustained activation of inflammatory cells, clear the wet mixture in treating chlamydial infection stasis anti-sperm immune infertility may be through the mechanism increased apoptotic factor Fas/Fas-L expression, reduced anti-apoptotic factor Bcl-2, thereby promoting apoptosis of inflammatory cells, inhibit inflammation of the uterine tissue injury, played a role in protecting the uterus fallopian tubes, thereby increasing the pregnancy possible.4. Qingshihuayu mixtures can significantly reduce the circulation of the inflammatory response in rats, uterine tubal tissue congestion, edema, inflammatory exudate, and significantly reduced adhesion, promoting tissue repair.
|
PDF Full Text Request