Research On The The Ischemic Tolerance And Metabolic Of Blood Lipid Of The Second Filial Generation Of MCAO-model With Blood-stasis-due-to-qi-deficiency Syndrome

Objective:To study whether there are any differences on the ischemic tolerance between the second filial generation (F2) of the middle cerebral artery occlusion (MCAO) model rats with blood-stasis-due-to-qi-deficiency syndrome (BSDTQDS) and the normally contemporary SD rats. And next to observe whether there were metabolic disorder of blood lipid in the F2 of MCAO- model rats with BSDTQDS, and whether the expression of APOE in plasma of the F2 of MCAO- model rats with BSDTQDS were significantly difference with the normally contemporary SD rats. To Research whether there are more risk factors in ischemic stroke in the second generation of MCAO- model rats with BSDTQDS than normally contemporary rats.Method:Part 1:The F2 rats were born through the First filial generation (F,) rats which were born through mating between male/female SD rats after BSDTQD syndrome molding and MCAO apoplexy molding, and such subjects were made syndrome molding once again to produce 10 second generation of syndrome experimental group(SEG) and compared with 10 normally contemporary SD rats by the same syndrome molding in syndrome controlled group(SCG) and other 10 normally contemporary SD rats without any intervention involved in blank controlled group(BCG) to compare changes in qi-deficiency syndrome grading, blood-stasis syndrome grading and related ethology index like open-field test, net-climbing.Part 2:In this part, ischemic preconditioning (IP) was induced by ligation of dual common carotid artery 3 min. Middle cerebral artery occlusion (MCAO) was induced by intraluminal filament for 2h after IP 3d. drug intervention was induced by Naoxintong administration through esophagus once a day. sham operation group was induced by Pretreatment Of sham Surgery.20 the F2 of MCAO- model rats with BSDTQDS and 20 SD rats were included and randomly divided in to 4 groups:normally group (normally +IP+MCAO). the F2 group (F2+IP+MCAO). drug intervention group (F2 +drug-treated+IP+MCAO). sham operation group (SS+MCAO). At 22h perfusion end Point, the neurologic deficit score, histopathological changes with HE staining and the expression of BDNF,Bax,Bcl-2 and ERK by using immunohistochemistry method were evaluated in each group.Part 3:The rats were divided into two groups randomly:model group (10 the F2 of MCAO- model rats), normal group (10 normally contemporary SD rats). The two group rats without any intervention were took the blood to do examinations as follow:â‘ the levels of triglyceride(TG),total cholesterol (TC),high density lipoprotein cholesterol (HDL-c),low density lipoprotein cholesterol(LDL-c) in blood.â‘¡analyze the atherogenic index of plasma (AIP) and Lipid Comprehensive index (LCI).â‘¢the expression of Apolipoprotein E (APOE) in plasma were examined by real-time reverse transcription-polymerase chain reaction.Result:Part1:Before being molded, qi-deficiency syndrome grading and related ethology index of SEG showed statistically significant difference in comparison with others, meanwhile blood-stasis syndrome grading showed no statistically significant difference among them. Having being molded, qi-deficiency syndrome grading, blood-stasis syndrome grading and related ethology index of SEG showed statistically significant difference in comparison with others.Part2:the neurologic deficit scores of the F2 +IP+MCAO group were significantly higher than that of the normally +IP+MCAO group and the F2 + drug -treated +IP+MCAO group. Meanwhile the expression of BDNF,Bcl-2 and ERK in the ischemic brain tissue of of the F2+IP+MCAO group were significantly less than that of the normally +IP+MCAO group and the F2+ drug -treated +IP+MCAO group, and the expression of Bax in the ischemic brain tissue of the F2 +IP+MCAO group were significantly higher than that of the normally +IP+MCAO group and the F2+drug -treated +IP+MCAO group.Part3:There were statistically significant difference in the levels of blood lipid(TG,TC,HDL-c,LDL-c)and the expression of APOE in plasma between the F2 of MCAO- model rats and the normally contemporary SD rats. Meanwhile, the atherogenic index of plasma and Lipid Comprehensive index of the F2 of MCAO- model rats were significantly higher than the normally contemporary SD rats.Conclusion:1. The trend of qi-deficiency syndrome at the F2 of MCAO- model rats with BSDTQDS are more than that of the normally contemporary SD rats. What's more, the F2of MCAO- model rats with BSDTQDS could be likely led by molding of BSDTQDS to more serious BSDTQDS manifestations than normally contemporary SD rats.2. Comparing with the the normally contemporary SD rats, the ischemic tolerance of the F2 of MCAO- model rats with BSDTQDS were reduced, and the risk of ischemic were increased.3. ERK signal transduction pathway and the expression of it's downstream target genes (such as BDNF,Bax and Bcl-2)might play important role in induction of the change of the ischemic tolerance in the F2 of MCAO- model rats with BSDTQDS.4. Naoxintong can improve the the ischemic tolerance of the F2 of MCAO-model rats with BSDTQDS.5. There were metabolic disorder of blood lipid in the F2 of MCAO- model rats with BSDTQDS.6. The expression of APOE in plasma of the F2 of MCAO- model rats with BSDTQDS were significantly higher than that of the normally contemporary SD rats.