Construction And Characterization Of Fully Human Anti-Her2 Antibodies

Objective:Her2 is a tyrosine kinase activity transmembrane glycoprotein, and its overexpression has relationship with tumor formation, the degree of malignancy and prognosis. In this study, we aim to prepare fully human anti-HER2 antibodies with potent antitumor activity. Methods:We constructed a large-capacity human ScFv phage antibody library and selected ScFv specific for HER2. Then the antibody expression vectors were constructed and anti-Her2 antibodies were expressed in CHO cells. Finally, we investigated the in vitro antitumor effects of these anti-Her2 antibodies on breast cancer cell lines. Results:Anti-Her2 antibodies could effectively inhibit the growth of Her2 high expresstion breast cancer cell lines SK-BR-3 and BT-474 in vitro. Conclusion:The present study provides several fully human anti-Her2 antibodies with potent antitumor activity and these anti-Her2 antibodies may serve as potential therapeutic agents for breast cancer.