| This article mainly studied chemical-physical indicators, lactation charactor, correlation between milk yield and milk composition, correlation between milk yield and body measurement, polymorphism of PRL and PRLR gene of Yili horse New Kirgiz horse and Hybrid horse in the Zhaosu Stud. The result is as follow:Chemical-physical indicators:Content of total protein and IgG in colostrum between Yili horse and Fl hybridization mare of new Kirghiz horse and Yili horse, protein in milk of Yili horse New Kirgiz horse and Hybrid horse, the type and content of amino acids in milk of Yili horse were compared and analyzed. The results showed that:(1)Protein in colostrum of Yili horse and hybrid horse was higher than that in milk, and gradually close to milk with the extension of lactation, and decreased dramatically in 12h after foaling. (2) Protein in Yili horse colostrum were different with hybrid horse at the same lactation period, as most measured values of Yili horse were higher than hybrid horse, and the difference was gradually narrowing with extended lactation period. (3) Protein in colostrum of Yili horse after foaling in 4 h were extremely significantly higher than that in hybrid horse (P<0.01), significantly higher than that in hybrid horse after foaling in 72 h (P<0. 05).(4) The difference of IgG content in Hybrid horse and Yili horese after foaling at given time internal, but the IgG content of Yili horse was higher than Hybrid horse. Contents of IgG declined sharply after foaling within 6h, so it is particularly important to let new foal eat colostrum as soon as possible.(5) Protein of three mare breeds was non-significant. (6) Protein of every breeds was non-significant in the lactation period.(7) Content and ratio of amino acids in mare's milk was appropriate. (8)Milk of Yili hores was rich of His, Glu, Asp, Met, Arg,Fat in colostrum between Yili horse and Fl hybridization mare of new Kirghiz horse and Yili horse, fat in milk of Yili horse New Kirgiz horse and Hybrid horse, the type and content of fatty acids in milk of Yili horse were compared and analyzed. The results showed that:(1)Fat in colostrum of Yili horse and hybrid horse reached maximum value at 24h after parturition, and were were stable after foaling in 96 h.(2) Trend in colostrum of two horse breed were first increased and then decreased, and change Interval of Yili horse was bigger than Hybrid horse. (3) Fat of three mare breeds was non-significant. (4) Fat of every breeds has some difference between different moments in the lactation period. (5) The content of unsaturated fatty acids of mare's milk was higher than other common dairy. (6) Mare's milk is rich in medium-chain fatty acids, so it have some health and medical capabilities. (7) There is a certain amount of OBCFA in mare's milk, especially in the colostrum. (8)Mare's milk was superior to other common dairy, because it was rich of EFA.Ash in colostrum between Yili horse and Fl hybridization mare of new Kirghiz horse and Yili horse, ash in milk of Yili horse New Kirgiz horse and Hybrid horse, minerals in colostrum of Yili horse New Kirgiz horse and Hybrid horse were compared and analyzed. The results showed that:(1)Ash in colostrum of Yili horse and hybrid horse was higher than that in milk, and gradually close to milk with the extension of lactation, and decreased dramatically in 12h after foaling.(2)Ash in Yili horse colostrum were different with hybrid horse at the same lactation period, as most measured values of Yili horse were higher than hybrid horse, and the difference was gradually narrowing with extended lactation period.(3)Ash in colostrum of Yili horse was significantly higher than that in hybrid horse after foaling in 72 h (P<0.05). (4) Ash of three mare breeds was non-significant. (5) Ash of every breeds was non-significant in the lactation period.(6) Content of minerals was less than other common dairy, but the ratio between minerals in mare's milk was appropriate.Density, non-fat solids, electrical conductivity in colostrum between Yili horse and Fl hybridization mare of new Kirghiz horse and Yili horse, density, non-fat solids, electrical conductivity in milk of Yili horse New Kirgiz horse and Hybrid horse were compared and analyzed. The results showed that:(1) Density, non-fat solids, electrical conductivity in colostrum of Yili horse and hybrid horse was higher than that in milk, and gradually close to milk with the extension of lactation.(2) Density, non-fat solids, electrical conductivity in Yili horse colostrum were different with hybrid horse at the same lactation period, as most measured values of Yili horse were higher than hybrid horse, and the difference was gradually narrowing with extended lactation period.(3) Density in colostrum of Yili horse significantly higher than that in hybrid horse after foaling in 72 h (P<0.05). (4) Non-fat solids decreased dramatically in 12h after foaling.(5) Density, non-fat solids of three mare breeds was non-significant. (5) Density, non-fat solids of every breeds was non-significant in the lactation period.(6) Variation of electrical conductivity of Yili horse was different of the Yili horse, and the absolute value of Yili horse was high.Regulation of lactation and Correlation:Milk yield and body measurement of Yili horse New Kirgiz horse and Hybrid horse was determined, and correlation between milk yield and milk composition, body measurement was analyzed. The results showed that:(1)The milk yield of New Kirgiz horse was extremely significantly higher than Hybrid horse,and Hybrid horse was extremely significantly higher than Yili horse. (2)The regulation of lactation of New Kirgiz horse was first high to low. The regulation of lactation of Hybrid horse, pre relatively flat and decreasing late was similar to Yili horse. Milk yield of three mare breed decrease fast in 80d after parturition. (3)Comparing with Yili horse and New Kirgiz horse, lactation charactor of Hybrid horse was unstable. (4)There is a strong positive correlation between milk yield and content of fat, non-fat solids, protein. Correlation between milk yield and fat percentage was very strong positive, no correlation between milk yield and protein percentage,and negative correlation between milk yield and non-fat solids percentage. (5) There is a strong positive correlation between milk yield and height at withers,body length,heart girth,circumference of cannon bone. Correlation between milk yield and chest girth ratio, trunk index was very strong positive, no correlation between milk yield and body length ratio, shank circumference ratio.Polymorphism of PRL and PRLR gene and correlation:PRL and PRLR gene polymorphism of three breeds were determined, and genotype frequency distribution, base sequence of different genotypes, correlation between polymorphism and milk yield was analyzed. The results showed that:(1)Polymorphism of PRL exon 5 was detected in the 4 genotypes which was studied, but PRL exon 4 was not。Mutation of PRL exon 5 occurred at 591bp(T→G)and 654bp(C→T)。By analyzing codon of amino acid, we found that mutation do not cause the change of amino acid.(2)Polymorphism of PRLR exon 4 was detected in the 2 genotypes which was studied, but PRL exon 3,10 was not.Mutation of PRL exon 4 occurred at 156bp(C→T)。By analyzing codon of amino acid, we found that mutation do not cause the change of amino acid。(3) There were significant and extremely significant differences between milk yield of different genotypes of Yili horse, but there were no differences of New Kirgiz horse and Hybrid horse. |
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