AMPA Receptor Tyrosine Phosphorylation Body Hypoxic Response

Aim: (1) The purpose of this study is to examine whether protein tyrosine kinase (PTK) and AMPA receptor in the nucleus tractus solitarius (NTS) and nucleus ambiguus were involved in the regulation of normoxia and hypoxia respiratory response by microinjection of PTK inhibitor and non-NMDA receptor antagonist; (2) To observe the change of number of phosphotyrosine immunopositive neurons in NTS and NA before and after hypoxia using immunohistochemical methods and whether tyrosine phosphorylation and GluR1 in postsynaptic membrane co-localize in NTS and NA neurons.Methods: 1. Electrophysiological experiments: The experiment was performed on anesthetized, paralyzed and artificially ventilated adult rabbits. The hypoxia respiratory response was produced by ventilating the animals with 10% O₂ (Nitrogen balanced). Phrenic nerve discharge was recorded as respiratory indicator. The NTS and NA were localized with extracellular recording of respiratory neurons. After microinjection of genistein (PTK inhibitor), daidzein (inactive inhibitor of PTK) and CNQX (inhibitor of NMDA receptor) into NTS or NA respectively, the changes of phrenic discharge and arterial blood pH under normoxia and hypoxia conditions were observed. 2. Fluorescent immunostaining:...