A New Testis-specific Nuclear Pore Protein Of Cdna Bs-63 Cloning, Characterization, And Expression

Spermatogenesis and spermiogenesis are a series of highly ordered process of molecular and cellular events. The spatial and temporal regulation of gene expression is very complicated. More and more cDNAs encoding sperm-related proteins involved in these two processes have been identified and characterized.In previous studies, a rabbit sperm tail protein with an estimated MW of 20KD was purified and designated as rSMP-B. Monoclonal antibodies (mAbs) were raised against the rSMP-B protein and demonstrated to interact with human as well as rat sperm proteins with an estimated MW of 70KD. These mAbs inhibited the penetration and fertilization of Zona-free hamster eggs by human sperm. Adult male rabbits were immunized with rSMP-B, their spermatogenesis was blocked at spermatid stage. It suggested that rSMP-B might play an important role in spermatogenesis and spermiogenesis. A 2.0Kb cDNA (RSD-1) was obtained by screening a rat testis A.gt11 cDNA expression library with mAbs against rSMP-B. Its sequence was determined and submitted to GenBank with accession number U26723.In the present study, a RSD-1 2.0Kb cDNA was used as a probe to screen full length cDNA from human testis λgt10 5'-stretch and λ ZAPII cDNA libraries respectively. 12 positive clones were obtained and confirmed by Southern blot. Three of them, H0620(2.7Kb), H0609(1.7Kb), H0324(1.9Kb) were sequenced. By homology comparison, high homology to each other (94-98%) and regional variation and deletion among them were uncovered. H0620 has a long...