Qingdao Amphioxus Developmental Related Genes Amphisom, Tropomyosin, Eif3k And Amphioxus Into Chemical Research

Amphioxus, a cephalochordate, shares a common ancestry with vertebrates. Although amphioxus and vertebrates split about 550million years ago and the amphioxus body structure is simpler than the vertebrates, it resembles the common ancestor of amphioxus and vertebrate. However, the amphioxus genome has not escaped evolution. In the 550 million years evolution, the genome of amphioxus is not duplicated but some independent genes are duplicated. And after duplication, the duplicated genes may survive and acquire novel function or they may lose in the genome, which is similar to the event that occurs in the vertebrates. So amphioxus is an important animal model for phylogenetic analysis including chordate developmental mechanisms and gene evolution.Here three genes (AmphiSom, Tropomyosin, eIF3k) were cloned from amphioxus, and the expression patterns and the phylogenetic analysis were performed. The amphioxus systematic evolution analysis on ferritin genes was also studied. These data give insight into the development mechanism and evolution of the amphioxus and vertebrates.A novel gene, AmphiSom, was identified in amphioxus Branchiostoma belcheri tsingtauense, and its sequence and developmental expression pattern were determined. In a large scale sequencing of an amphioxus neurula cDNA library, more than 5000 clones are analysed. Among them a partial sequence of AmphiSom is found. This sequence is 1200bp long. Analysis of the extended sequence with blastX in NCBI and the soft Clone, we obtain the whole open reading frame. Southern Blotting indicates that amphioxus might have only one copy of AmphiSom gene in its genome.The spatial and temporal expression pattern of AmphiSom is analysed by whole mount in situ hybridization followed by sectioning. The gene doesn't express itself before 9 hours after fertilized. And faint AmphiSom expression is first detected in late-gastrula stage. AmphiSom transcripts...