Cloning And Function Of Human Genes Sec14l3, Acad10 And Bbs4v2

A large full-length gene bank has been contructured through large-scale cDNA cloning and sequencing. With kinds of bioinformatical analysis, we try to select novel human genes that maybe related to crucial pathways or human diseases. Based on these works we selected three novel genes as SEC14L3, ACAD10 and BBS4v2 for further research.The cDNA sequece of SEC14L3 is 2082 base pairs in length with an open reading frame encoding a 400 amino acids protein. Analysis of protein sequece shows that SEC14L3 contains two important domains as CRAL-TRIO and GOLD. As SEC14L3 is highly homologous to human SPF/TAP, we think it maybe a new member of CRAL-TRIO protein family. The SPF/TAP can regulate the synthesization of cholesterol and the metabil metabolization of α -Tocophel in wVo.lt is predicted that SEC14L3 maybe have a similar function like SPF/TAP. Through pEGFP-fusion protein expression and tissue distrubition analysis, we find that SEC14L3 is a cytoplasmic protein and it mostly expressed in liver. We haven't found the transfer from cytoplasm to nucleolus in the existence of biophysical concentration of α -tocophel compared to SPF/TAP. By simulation of protein structure and amino-acids analysis we find there are significant difference in the key amino acid and...